Diagnostic and prognostic potential of miR-21, miR-29c, miR-148 and miR-203 in adenocarcinoma and squamous cell carcinom
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SHORT REPORT
Open Access
Diagnostic and prognostic potential of miR-21, miR-29c, miR-148 and miR-203 in adenocarcinoma and squamous cell carcinoma of esophagus Renata Hezova1,2, Alena Kovarikova1, Josef Srovnal3, Milada Zemanova4, Tomas Harustiak5, Jiri Ehrmann6, Marian Hajduch3, Marek Svoboda2, Milana Sachlova2 and Ondrej Slaby1,2*
Abstract Background: Esophageal cancer is the malignant tumor with very poor prognosis and increasing incidence often diagnosed at very late stage, so the prognosis of affected patients is unsatisfactory, despite the development of therapeutic option such as surgery, chemotherapy and radiotherapy. Consequently, there is a great need for biomarkers to allow a tailored multimodality approach with increased efficiency. Altered expression of microRNAs has been reported in wide range of malignancies, including esophageal cancer. The aim of this study was to examine the expression levels of candidate microRNAs in esophageal cancer and evaluate their diagnostic and prognostic potential. Findings: Using quantitative real-time PCR, expression levels of 9 candidate microRNAs were examined in 62 tissue samples, 23 esophageal adenocarcinomas, 22 esophageal squamous cell carcinomas and 17 adjacent esophageal mucosa samples. MicroRNA expression levels were further analyzed in regards to clinico-pathological features of esophageal cancer patients. We observed significantly decreased levels of miR-203 and increased levels of miR-21 in adenocarcinoma tissues when compared to normal mucosa. MiR-29c and miR-148 indicated good ability to distinguish between histological subtypes of esophageal cancer. MiR-203 and miR-148 were linked to disease-free survival and overall survival in esophageal adenocarcinoma patients, and miR-148 also in esophageal squamous cell carcinoma patients. Conclusions: Our data suggest that altered expression of miR-21, miR-29c, miR-148 and miR-203 are related to neoplastic transformation and progression of the disease and these microRNAs could serve as a potential diagnostic and prognostic biomarkers in esophageal cancer. Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/ vs/4646922201567057
Findings Background
Esophageal cancer (EC) is the seventh most common cancer worldwide and the sixth most common cause of cancer death [1]. There are two main types of esophageal cancer – adenocarcinoma and squamous cell carcinoma with distinct etiology and epidemiology. * Correspondence: [email protected] 1 Molecular Oncology II – Solid Cancers, Molecular Medicine Central European Institute of Technology, Masaryk University, Brno, Czech Republic 2 Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno 656 53, Czech Republic Full list of author information is available at the end of the article
Esophageal adenocarcinoma (EAC) is one of the fastest rising cancers in Western society. Incidence has increased by 600% within the last 30 years [2]. The reason of increasing incidence is not
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