Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio par
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Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND) Lean Huat Yu 1 & Cindy Shuan Ju Teh 2 & Kien Pong Yap 1 & Kwai Lin Thong 3 Received: 1 June 2020 / Accepted: 23 September 2020 / Published online: 26 September 2020 # Springer Nature Switzerland AG 2020
Abstract
A unique strain of Vibrio parahaemolyticus (designated as VPAHPND) causes acute hepatopancreatic necrosis disease (AHPND), a deadly bacterial disease associated with mass mortality in cultured shrimps since 2009. AHPND is responsible for severe economic losses worldwide, causing multimillion-dollar loss annually. Because of the rapid and high mortality rates in shrimps, substantial research has been carried out to develop rapid detection techniques. Also, recent technological advances such as the nextgeneration sequencing (NGS) have made it possible to elucidate relevant information about a pathogen in a single assay. This review summarizes the current research pertaining to VPAHPND, focusing on diagnosis and contribution of NGS technologies in the genomic studies of AHPND. Keywords Acute hepatopancreatic necrosis disease . Vibrio parahaemolyticus . Diagnostics . Next-generation sequencing
Introduction Shrimp aquaculture is the fastest-growing production sector in the fishery industry, and it plays a big role in worldwide economy, market demands and employment (FAO 2016). The
* Cindy Shuan Ju Teh [email protected]
1
Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
3
Nanocat Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia
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Aquaculture International (2020) 28:2547–2559
increases in market demands has led the shrimp aquaculture to develop into an important export-oriented food industry especially in Southeast Asia, generating billions of US dollars in export income annually (FAO 2016; GOAL 2018). As the shrimp industry expands, more issues and challenges are faced by farmers due to intensification of shrimp farming. Among these issues and challenges, shrimp diseases remain the most critical challenge as a limiting factor for the expansion of shrimp production (GOAL 2018). The shrimp industry is challenged with a plethora of pathogens such as infection with infectious hypodermal and haematopoietic necrosis (IHHN), infectious myonecrosis (IMN), white tail disease (WTD), taura syndrome (TS), yellow head disease (YHD) and white spot disease (WSD), and it had caused a loss of approximately US$ 15 billion in revenue over the past 15 years. In addition, lately a bacterial disease known as the acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio parahaemolyticus, had superseded other diseases, causing high and rapid mortalities in all types of cultured shrimps and economical loss incurred more US$ 1 billion, annual
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