Difference Gel Electrophoresis Methods and Protocols
This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters inDifference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis
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Kay Ohlendieck Editor
Difference Gel Electrophoresis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Difference Gel Electrophoresis Methods and Protocols
Edited by
Kay Ohlendieck Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland
Editor Kay Ohlendieck Department of Biology Maynooth University, National University of Ireland Maynooth, Co. Kildare, Ireland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7267-8 ISBN 978-1-4939-7268-5 (eBook) DOI 10.1007/978-1-4939-7268-5 Library of Congress Control Number: 2017952363 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The aim of this new edition of Difference Gel Electrophoresis is to provide a comprehensive update of this key method of gel-based proteomics. Two-dimensional gel electrophoresis is one of the most frequently used protein separation techniques in modern biochemistry and proteomics. Difference Gel Electrophoresis employs the direct labeling of proteomes with fluorescent dyes prior to large-scale gel electrophoretic separation enabling highthroughput comparative analyses. Labeling of two or more samples with different dyes followed by separation on the same gel system eliminates gel-to-gel variations, making this advanced technique a robust and highly suitable method for proteomic profiling surveys with diverse biological applications
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