Microchip Capillary Electrophoresis Protocols
In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Writte
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Ann Van Schepdael Editor
Microchip Capillary Electrophoresis Protocols
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Microchip Capillary Electrophoresis Protocols Edited by
Ann Van Schepdael Pharmaceutical Analysis, KU Leuven, Leuven, Belgium
Editor Ann Van Schepdael Pharmaceutical Analysis KU Leuven Leuven, Belgium
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2352-6 ISBN 978-1-4939-2353-3 (eBook) DOI 10.1007/978-1-4939-2353-3 Library of Congress Control Number: 2015931079 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Cover image courtesy of Bart Heleven Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Microchip capillary electrophoresis (MCE) has evolved from capillary electrophoresis through further miniaturization, providing improvements in speed and sample requirements as well as the possibility to perform more complex and highly integrated analyses. MCE may be well investigated in the scientific literature, yet it is a rapidly maturing technique, and detailed validated protocols are not widely described yet. This book is intended to be a working guide to the operation of microchip capillary electrophoresis. It explains step by step how to fabricate and operate microchips in electrophoresis as well as electrochromatography mode. It addresses some small molecule as well as biomolecule applications. Furthermore, various detection modes as well as sample preparation approaches are described. In analogy with capillary electrophoresis, MCE also knows a large variety of application fields such as dr
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