Protein Electrophoresis Methods and Protocols
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and under
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protein Electrophoresis Methods and Protocols Edited by
Biji T. Kurien and R. Hal Scofield University of Oklahoma Health Sciences Center Oklahoma Medical Research Foundation Department of Veterans Affairs Medical Center Oklahoma City, OK, USA
Editors Biji T. Kurien University of Oklahoma Health Sciences Center Oklahoma Medical Research Foundation Department of Veterans Affairs Oklahoma City, OK, USA
R. Hal Scofield University of Oklahoma Health Sciences Center Oklahoma Medical Research Foundation Department of Veterans Affairs Medical Center Oklahoma City, OK, USA
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-820-7 e-ISBN 978-1-61779-821-4 DOI 10.1007/978-1-61779-821-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012936829 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Cover illustration: A vertical air cooled starch gel analyzing haptoglobin polymers (Figure 13 in Chapter 1; gel run started at 3:50 PM on Tuesday, June 3rd and stopped at 10:50 AM on Wednesday, June 4th, 1958) Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface We previously edited a book similar to this one in which methods and protocols were presented for protein blotting. The present work concentrates upon methods for electrophoresis. Again, each chapter outlines a specific electrophoretic variant and gives stepby-step instructions. Most often, the instructions found in a typical paper do not allow a laboratory scientist to easily reproduce a technique. Sometimes it seems to us that technique protocols are like food recipes—if one does not already know how to cook the dish, or a very similar dish, then recipes are not very helpful. The instructions given in this textbook are much more detailed than those typically found in research papers (or recipes). We imagine that one will be able to open this textbook in one’s lab, and by following the steps given, perform a technique new to that lab without too much difficulty. We hope that investigators will find this is the case. We have elected to open this textbook with a
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