Different bacterial host-based lux reporter array for fast identification and toxicity indication of multiple metal ions
- PDF / 1,371,325 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 71 Downloads / 148 Views
RESEARCH PAPER
Different bacterial host-based lux reporter array for fast identification and toxicity indication of multiple metal ions Yuchen Su 1 & Chunlan Liu 1 & Xuemei Jiang 2 & Weili Wei 1 Received: 13 June 2020 / Revised: 15 August 2020 / Accepted: 4 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Although luminescent bacteria-based bioluminescence inhibition assay has been widely used in the toxicity assessment of environmental pollutants, the response of a luminescent bacterium usually lacks specificity to a target analyte. Recently, some specific analyte inductive promoters were fused to the lux genes for the purpose of selective bioluminescent sensing, and suits of specific promoters were fused to lux genes to compose a bioluminescent array sensor for simultaneous identification of multiple analytes. However, specific promoter-based methods still suffer from drawbacks including limited selectivity, slow responding time, expensive to construct different promoters involved plasmids, and laborious to find new promoters. Herein, we proposed a novel strategy to construct a lux reporter array sensor by directly transforming the natural lux genes in different bacterial hosts without the involvement of any specific promoters. Due to the distinct pathways of signal production, the responding time of the current different bacterial host (DBH)-based lux reporter array has nearly an order of magnitude faster than with specific promoter-based methods. The DBH-based lux reporter array was successfully used for simultaneous identification, quantification, and toxicity/bioactivity assessment of multiple metal ions. Obviously, all the chemical synthetic material-based metal ion sensing methods cannot simultaneously achieve analysis and toxicity evaluation. This approach possessed additional advantages of facile construction, easy operation, high selectivity, fast response, and strong adaptability to other analytes. Keywords Luminescent bacteria . Sensor array . Pattern recognition . Metal ions . Toxicity
Introduction Natural luminescent bacteria such as Vibrio fischeri can spontaneously emit visual light by proceeding the lux gene responsible bioreactions [1, 2]. Plenty of researches showed that the bacterial bioluminescence can be affected by exogenous substances [3, 4]. On account of the advantages of stability, sensitivity, and accuracy, the bioluminescence inhibition assay based on V. fischeri is being used as International Standardization Organization (ISO)-approved method [5] for Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02943-8) contains supplementary material, which is available to authorized users. * Weili Wei [email protected] 1
School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China
2
Bioengineering College, Chongqing University, Chongqing 400044, China
toxicity tests [6–8]. Despite the wide application, such single natural luminescent bacterium-based assay could only provide the i
Data Loading...
