Differential expression of microRNAs in porcine parvovirus infected porcine cell line
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Differential expression of microRNAs in porcine parvovirus infected porcine cell line Xinqiong Li1†, Ling Zhu1,2†, Xiao Liu1, Xiangang Sun1, Yuanchen Zhou1, Qiaoli Lang1, Ping Li1, Yuhan Cai1, Xiaogai Qiao1 and Zhiwen Xu1,2*
Abstract Background: Porcine parvovirus (PPV), a member of the Parvoviridae family, causes great economic loss in the swine industry worldwide. MicroRNAs (miRNAs) are a class of non-protein–coding genes that play many diverse and complex roles in viral infections. Finding: Aiming to determine the impact of PPV infections on the cellular miRNAome, we used high-throughput sequencing to sequence two miRNA libraries prepared from porcine kidney 15 (PK-15) cells under normal conditions and during PPV infection. There was differential miRNA expression between the uninfected and infected cells: 65 miRNAs were upregulated and 128 miRNAs were downregulated. We detected the expression of miR-10b, miR-20a, miR-19b, miR-181a, miR-146b, miR-18a, and other previously identified immune-related miRNAs. Gene Ontology analysis and KEGG function annotations of the host target genes suggested that the miRNAs are involved in complex cellular pathways, including cellular metabolic processes, immune system processes, and gene expression. Conclusions: These data suggest that a large group of miRNAs is expressed in PK-15 cells and that some miRNAs were altered in PPV-infected PK-15 cells. A number of microRNAs play an important role in regulating immune-related gene expression. Our findings should help with the development of new control strategies to prevent or treat PPV infections in swine.
Background Porcine parvovirus (PPV) is a major cause of reproductive failure in swine (Sus scrofa, ssc), where infection is characterized by early embryonic death, stillbirths, fetal death, and delayed return to estrus [1]. Additionally, PPV is associated with porcine postweaning multisystemic wasting syndrome (PMWS) and diarrhea, skin disease, and arthritis in swine [1, 2]. Even though inactivated and attenuated vaccines are widely used, the PPV-associated diseases nevertheless cause serious economic losses to the swine industry worldwide [3]. As virus replication is highly dependent on the host cell, cellular microRNA (miRNA) modification of the complex cellular regulatory networks can greatly influence viral reproduction and pathogenesis. Therefore, determining the consequences of PPV infections on cellular gene regulatory networks is urgent. * Correspondence: [email protected] † Equal contributors 1 Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, China 2 Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, China
miRNAs are involved in post-transcriptional regulation of gene expression in animals, plants, and some DNA viruses. miRNAs act as regulators, inhibiting the expression of specific mRNAs by recognizing partial complementary sites in a targeted mRNA
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