MicroRNA regulation in hypoxic environments: differential expression of microRNAs in the liver of largemouth bass ( Micr
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MicroRNA regulation in hypoxic environments: differential expression of microRNAs in the liver of largemouth bass (Micropterus salmoides) Jun Long Sun & Liu Lan Zhao & Kuo He & Qiao Liu & Jie Luo & Dong Mei Zhang & Ji Liang & Lei Liao & Ji Deng Ma & Song Yang
Received: 9 April 2020 / Accepted: 8 September 2020 # Springer Nature B.V. 2020
Abstract Environmental changes in intensive aquaculture commonly lead to hypoxic stress for cultured largemouth bass (Micropterus salmoides). To better to
understand the hypoxic stress response mechanisms, the miRNA expression profiles of the livers of largemouth bass exposed for 24 h to three different dissolved oxygen
Jun Long Sun and Liu Lan Zhao contributed equally to this work. Highlights 1. Acute hypoxia significantly affected miRNA expression patterns. 2. Revealed two new important hypoxia-related signal transduction pathways: the MAPK signaling pathway and the phosphatidylinositol signaling system. 3. Provided several miRNA-mRNA interaction networks under hypoxia. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10695-020-00877-7) contains supplementary material, which is available to authorized users. J. L. Sun : L. L. Zhao : K. He : Q. Liu : J. Luo : D. M. Zhang : J. Liang : L. Liao : J. D. Ma : S. Yang (*) College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 Sichuan, China e-mail: [email protected]
J. L. Sun e-mail: [email protected] L. L. Zhao e-mail: [email protected] K. He e-mail: [email protected] Q. Liu e-mail: [email protected] J. Luo e-mail: [email protected]
D. M. Zhang e-mail: [email protected] J. Liang e-mail: [email protected] L. Liao e-mail: [email protected] J. D. Ma e-mail: [email protected] J. L. Sun State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan University, Haikou 570228 Hainan, China
Fish Physiol Biochem
levels (7.0 ± 0.2 mg/L as control, 3.0 ± 0.2 mg/L and 1.2 ± 0.2 mg/L) were compared. In this study, a total of 266 known miRNAs were identified, 84 of which were differentially expressed compared with the control group. Thirteen of the differentially expressed miRNAs (miR15b-5p, miR-30a-3p, miR-133a-3p, miR-19d-5p, miR1288-3p, miR456, miR-96-5p, miR-23a-3p, miR-23b5p, miR-214, miR-24, miR-20a-3p, and miR-2188-5p) were significantly enriched in VEGF signaling pathway, MAPK signaling pathway, and phosphatidylinositol signaling system. These miRNAs were significantly downregulated during stress, especially after a 4-h exposure to hypoxia. In contrast, their target genes (vegfa, pla2g4a, raf1a, pik3c2a, clam2a, inpp1, pi4k2b, mtmr14, ip6k, itpkca, map3k7, and Jun) were significant upregulated after 4 h of hypoxic stress. Moreover, two potential hypoxia-tolerance signal transduction pathways (MAPK signaling pathway and phosphatidylinositol signaling system) were revealed, both of which may play important roles in responding to acute hypoxic stress. We see that
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