Discussion of the promising effect of electroacupuncture on cognitive improvement in D-galactose-induced aging rats base
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Basic Study
Discussion of the promising effect of electroacupuncture on cognitive improvement in D-galactose-induced aging rats based on NLRP3-ASC-Caspase-1 signaling pathway 基于NLRP3-ASC-Caspase-1信号通路探讨电针对 D-半乳糖诱导衰老大鼠认知的改善效应 Zhao Fei-yi (赵非一)1, Zhao Ying-xia (赵英侠)2, Yan Hai-xia (燕海霞)2, Xu Hong (许红)3, Hong Yu-fang (洪钰芳)3, Xu Yan (徐燕)1 1 Department of Nursing, School of International Medical Technology, Shanghai Sanda University, Shanghai 201209, China 2 School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 3 Shanghai Municipal Hospital of TCM Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China
Abstract Objective: To investigate the effect of electroacupuncture (EA) on cognitive function in D-galactose (D-gal)-induced aging rats, and the correlation between the effect and nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3)-ASC-Caspase-1 signaling pathway. Methods: Forty-six male Sprague-Dawley (SD) rats were randomly divided into a control group (n=10), a model group (n=12), an EA-7 d group (n=12) and an EA-21 d group (n=12). Except the control group, the other three groups received 42 consecutive days of intraperitoneal injection of D-gal to establish aging rat models with cognitive dysfunction. The control group received the same amount of normal saline via intraperitoneal injection. Two EA groups were given EA therapy for 21 consecutive days (began from the 22nd day of modeling) or 7 consecutive days (began from the 36th day of modeling) accordingly at Dazhui (GV 14), Baihui (GV 20), Shenshu (BL 23) and Zusanli (ST 36). After modeling/ intervention, all four groups received behavioral evaluations by Morris water maze (MWM) test, novel object recognition (NOR) test and step-down passive avoidance (SDPA) test followed by the Western Blot (WB) detection of the expression levels of hippocampal NLRP3 inflammasome-associated proteins NLRP3, ASC and Caspase-1. Results: MWM (place navigation test, PNT) results showed that the escape latency in the model group was significantly longer than that in the other three groups (P0.05). From the 2nd day to the 4th day of the test, there was no significant difference between the EA-21 d group and the control group (P>0.05) in the escape latency; the escape latency was significantly shorter in the EA-21 d group than in the model group and the EA-7 d group (P
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