Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena
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RESEARCH ARTICLE
Open Access
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena Rachel Goddard1*† , Andrew Steed1†, Catherine Chinoy1, Jéssica Rosset Ferreira2, Pedro Luiz Scheeren3, João Leodato Nunes Maciel3, Eduardo Caierão3, Gisele Abigail Montan Torres3, Luciano Consoli3, Flavio Martins Santana3, José Mauricio Cunha Fernandes3, James Simmonds1, Cristobal Uauy1, James Cockram4 and Paul Nicholson1
Abstract Background: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. Results: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terenalike haplotype for more than one of the identified heading stage QTL. (Continued on next page)
* Correspondence: [email protected] † Rachel Goddard and Andrew Steed contributed equally to this work. 1 Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons lice
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