Influence of obstetric factors on osteogenic potential of umbilical cord-derived mesenchymal stem cells
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Influence of obstetric factors on osteogenic potential of umbilical cord-derived mesenchymal stem cells Letizia Penolazzi1, Renata Vecchiatini1,2, Stefania Bignardi3, Elisabetta Lambertini1, Elena Torreggiani1, Alessandro Canella1, Tiziana Franceschetti1,4, Giorgio Calura2, Fortunato Vesce3 and Roberta Piva*1 Address: 1Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Italy, 2Department of MedicoSurgical Disciplines of Communication and Behaviour, University of Ferrara, Italy, 3Department of Biomedical Sciences and Advanced Therapies, Section of Obstetric and Gynaecological Clinic, Azienda Ospedaliero-Universitaria S Anna, Ferrara, Italy and 4Department of Reconstructive Science, University of Connecticut Health Center, Farmington, Connecticut, USA Email: Letizia Penolazzi - [email protected]; Renata Vecchiatini - [email protected]; Stefania Bignardi - [email protected]; Elisabetta Lambertini - [email protected]; Elena Torreggiani - [email protected]; Alessandro Canella - [email protected]; Tiziana Franceschetti - [email protected]; Giorgio Calura - [email protected]; Fortunato Vesce - [email protected]; Roberta Piva* - [email protected] * Corresponding author
Published: 5 October 2009 Reproductive Biology and Endocrinology 2009, 7:106
doi:10.1186/1477-7827-7-106
Received: 12 August 2009 Accepted: 5 October 2009
This article is available from: http://www.rbej.com/content/7/1/106 © 2009 Penolazzi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Wharton's jelly from the umbilical cord is a noncontroversial source of mesenchymal stem cells (WJMSCs) with high plasticity, proliferation rate and ability to differentiate towards multiple lineages. WJMSCs from different donors have been characterized for their osteogenic potential. Although there is large evidence of WJMSCs plasticity, recently scientific debate has focused on MSCs selection, establishing predictable elements to discriminate the cells with most promising osteoprogenitor cell potential. In the present study a comparative study between the presence of osteoblastic markers and different parameters that pertain to both the newborn and the mother was performed. Umbilical cords were collected after all patients signed the informed consent and local ethical commettee approved the study. Obstetric parameters, including baby's gender and birth weight, mother's age at delivery, gestational stage at parturition and mode of delivery were examined. After characterization and expansion, WJMSCs were analyzed for two osteoblastic markers, alkaline phosphatase (ALP) activity, and the expression level of RUNX-2 transcription factor, and for their ability to deposit mineralized matrix aft
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