Diversity of Culturable Actinobacteria Producing Protease Inhibitors Isolated from the Intertidal Zones of Maharashtra,
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Diversity of Culturable Actinobacteria Producing Protease Inhibitors Isolated from the Intertidal Zones of Maharashtra, India Neha A. Shintre1 · Vaijayanti A. Tamhane2 · Ulfat I. Baig3 · Anagha S. Pund3 · Rajashree B. Patwardhan4 · Neelima M. Deshpande1 Received: 12 March 2020 / Accepted: 21 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Phylogenetic diversity of culturable actinobacteria isolated from the intertidal regions of west coast of Maharashtra, India was studied using 16S rRNA gene sequencing. Total of 140 actinobacterial isolates were obtained, which belonged to 14 genera, 10 families and 65 putative species with Streptomyces being the most dominant (63%) genus followed by Nocardiopsis and Micromonospora. Isolates were screened for production of extracellular protease inhibitors (PI) against three pure proteases viz. chymotrypsin, trypsin, subtilisin and a crude extracellular protease from Pseudomonas aeruginosa. Eighty percent of the isolates showed PI activity against at least one of the four proteases, majority of these belonged to genus Streptomyces. Actinobacterial diversity from two sites Ade (17° 52′ N, 73° 04′ E) and Harnai (17° 48′ N, 73° 05′ E) with varying anthropological pressure showed that more putative species diversity was obtained from site with lower human intervention i.e. Ade (Shannon’s H 3.45) than from Harnai (Shannon’s H 2.83), a site with more human intervention. However, in Ade, percentage of isolates not showing PI activity against any of the proteases was close to 21% and that in Harnai was close to 9%. In other words, percentage of PI producers was lower at a site with lesser human intervention.
Introduction Proteases are enzymes that catalyse proteolytic reactions and are involved in variety of biological processes like digestion, cell signalling or tumour formation [1–4]. They This article is present on a repository website and can be accessed on https://www.biorxiv.org/content/10.1101/2020.02.14.949438v2 Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02174-1) contains supplementary material, which is available to authorized users. * Neelima M. Deshpande [email protected] 1
Department of Microbiology, M.E.S. Abasaheb Garware College, Karve Road, Pune, Maharashtra 411004, India
2
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra 411007, India
3
Indian Institute of Science Education and Research, Pune (IISER-P), Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India
4
Department of Microbiology, Haribhai V. Desai College of Commerce, Arts and Science, Pune, Maharashtra 411002, India
are ubiquitously present across different plant, animal and microbial taxa. Two of the many roles of these enzymes in bacteria are to aid in bacterial pathogenesis [5, 6] and bacterial predation where they secrete hydrolytic enzymes in order to degrade prey species in the vicinity [7]. Protease inhibitors bi
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