Diversity and characterization of culturable haloalkaliphilic bacteria from two distinct hypersaline lakes in northern E
- PDF / 769,362 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 111 Downloads / 179 Views
ORIGINAL ARTICLE
Diversity and characterization of culturable haloalkaliphilic bacteria from two distinct hypersaline lakes in northern Egypt Mervat A. Arayes 1 & Mona E. M. Mabrouk 1 & Soraya A. Sabry 2 & Bahaa Abdella 3,4 Received: 13 June 2020 / Accepted: 18 September 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract Soda lakes characterized by salinity and elevated pH, these lakes represent a vast reservoir of novel microbial diversity of potential applications. In this article, two distinct salt lakes in northwestern Egypt were explored for the diversity of cultivable aerobic haloalkaliphilic bacteria. Physicochemical analyses revealed that samples possessed the characteristic features of alkaline hypersaline lakes (high pH, high Na+, and Cl− content with low Mg+2). The phenotypic characterization showed great diversification between members of the same genus. They were characterized phenotypically based on their cell and colony morphology, production of extra-cellular enzymes, some metabolic activities and antibiogram profile. Phylogenetic analysis was performed using 16S rRNA sequence. All strains were haloalkaliphilic belonging to phylum Firmicutes. They were affiliated to the genera Alkalibacillus, Salinicoccus, Gracilibacillus, Thalassobacillus, Halobacillus, Staphylococcus, and Bacillus. Surprisingly, Thalassobacillus species were only isolated from the sediment of Marsa-Matrouh lake (MML), while members of the genera Alkalibacillus, Salinicoccus and Staphylococcus were isolated from the sediment of Al-Hamra lake (AHL). Members of the genus Gracilibacillus were among the common community of the two lakes. Keywords Al-Hamra lake . Extremophiles . Marsa-Matrouh lake . Saline lakes
Abbreviations AHL Al-Hamra lake MML Marsa-Matrouh lake EPS Exopolysaccharides CCA Canonical correspondence analysis UPGMA Unweighted Pair Group Mean Averages
Electronic supplementary material The online version of this article (https://doi.org/10.2478/s11756-020-00609-5) contains supplementary material, which is available to authorized users. * Bahaa Abdella [email protected] 1
Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhur, Egypt
2
Botany & Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt
3
Department of Biological Sciences, Sunway University, Petaling Jaya, Selangor, Malaysia
4
Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh, Egypt
Introduction Haloalkaliphiles are extremophilic microorganisms capable of robust growth in environments characterized by high salinity and alkalinity (Sorokin and Kuenen 2005a, 2005b). They are mostly confined to naturally occurring soda lakes and soda deserts distributed around the world (Zhao et al. 2014). Microorganisms defined to be optimally grown at either at or above pHs 9, 9.5, or 10 are termed alkaliphiles (Horikoshi 2006; Krulwich and Ito 2013). Haloalkaliphiles are a subgroup of alkaliphiles that require both conditions of high alkalinit
Data Loading...
