Do Mesenchymal Stem Cells Modulate the Milieu of Reconstructed Bladder Wall?
- PDF / 1,540,809 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 9 Downloads / 183 Views
ORIGINAL ARTICLE
Do Mesenchymal Stem Cells Modulate the Milieu of Reconstructed Bladder Wall? Marta Pokrywczynska • Arkadiusz Jundzill • Magdalena Bodnar • Jan Adamowicz • Jakub Tworkiewicz • Lukasz Szylberg • Robert Debski Andrzej Marszalek • Tomasz Drewa
•
Received: 5 July 2012 / Accepted: 5 August 2013 / Published online: 22 August 2013 Ó The Author(s) 2013. This article is published with open access at Springerlink.com
Abstract To evaluate the mesenchymal stem cells (MSCs) influence on cytokines and matrix metalloproteinases (MMPs) expression in rat bladder wall regeneration. MSCs cultures from the bone marrow were established. Acellular matrices from the bladder submucosa were prepared. Bladders were reconstructed using cell-seeded (n = 5) and unseeded (n = 5) grafts. MSCs were injected into the bladder wall (n = 5), bladders were incised and MSCs were injected into the circulation
(n = 5) or were left intact (n = 5). Animals were killed after 3 months. Bladder histology and immunohistochemical staining of IL-2, IL-4, IL-6, IL-10, TNF-a, TGF-b1, IFN-c, MMP-2, and MMP-9 were done. Bladders reconstructed with cell-seeded grafts mimicked native tissue, while unseeded grafts revealed shrinkage and morphological irregularities. There were no morphological changes in bladders of other groups. Different pattern of cytokine and MMP expression was observed. Increased expression of anti-inflammatory cytokines and MMPs in bladder promotes detrusor regeneration.
M. Pokrywczynska (&) A. Jundzill J. Adamowicz J. Tworkiewicz T. Drewa Department of Tissue Engineering, Ludwik Rydygier Medical College in Bydgoszcz, Nicolaus Copernicus University in Torun, Karlowicza 24, 85-092 Bydgoszcz, Poland e-mail: [email protected]
Keywords Bladder regeneration Cytokines Matrix metalloproteinases Mesenchymal stem cells Tissue engineering
A. Jundzill Department of General and Vascular Surgery, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland
Introduction
M. Bodnar L. Szylberg A. Marszalek Department of Clinical Pathomorphology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland A. Marszalek Department of Pathology, Poznan University of Medical Sciences, Poznan, Poland R. Debski Department of Pediatric Hematology and Oncology, Bydgoszcz, Poland T. Drewa Department of Urology, Nicolaus Copernicus Hospital, Torun, Poland T. Drewa Department of Urology, Institute of Oncology, Kielce, Poland
The gold standard for bladder creation after radical cystectomy is the use of gastrointestinal segments. However, using bowel as a substitute is associated with complications (Nieuwenhuijzen et al. 2008). This encouraged research in tissue engineering for bladder reconstruction. The key idea of this approach is construction of the new bladder wall from autologous cells expanded in vitro and seeded on biodegradable scaffold followed by transplantation for the completion of the regeneration process (Atala et al. 2006; Drewa et al. 2009; Sharma et al. 2011)
Data Loading...
