Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals
- PDF / 3,901,433 Bytes
- 11 Pages / 612 x 797 pts Page_size
- 79 Downloads / 192 Views
Original Article
Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals Ji Wook Choi1,†, Young Jae Kim1,†, Jong Min Lee1,2, Jin-Ha Choi3, Jeong-Woo Choi3 & Bong Geun Chung 1,* Received: 23 April, 2020 / Accepted: 9 June, 2020 / Published online: 1 September, 2020 ⒸThe Korean BioChip Society and Springer 2020
Abstract Gold nanoparticles (AuNPs) have widely used for various biological applications, such as drug screening, photo-thermal therapy, and biosensing. In particular, the synthesis of AuNPs with narrow size distribution plays an important role in increasing the efficiency of nanoparticle-mediated biosensors. However, the conventional synthesis methods (e.g., citrate reduction method) still suffer from controlling the sizes of the nanoparticles. In this paper, we present the synthesis method of homogeneous AuNPs using a droplet-based microfluidic chip. Prior to experiments, we optimized the size of droplets using a simulation software for stable droplet generation. We demonstrated that the nanoparticles synthesized in our microfluidic chip system showed a narrower size distribution and a higher reproducibility compared to conventional batch synthesis. Furthermore, we observed that the signal of anti-horseradish peroxidase (HRP) was significantly enhanced by the droplet-based microfluidic chip. Therefore, our synthesis method of homogeneous AuNPs could play an important role in improving the efficiency of AuNPs-based sensing signals. Keywords: Droplet-based microfluidic chip, Gold nanoparticles, HRP detection 1
Department of Mechanical Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea 2 Division of Chemical Industry, Yeungnam University College, 170 Hyeonchung-ro, Nam-gu, Daegu 42415, Republic of Korea 3 Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea † These authors contributed equally. *Correspondence and requests for materials should be addressed to B.G. Chung ( [email protected])
Introduction Nanoparticles have attracted significant interest in past decades due to their unique physical, chemical, and optical properties1,2. Due to these excellent properties, nanoparticles have previously been used for a wide range of applications in bio-sensing3,4, chemotherapy5,6, and diagnosis of diseases7,8. In particular, noble metal-based nanoparticles have allowed for development of bio-sensing with improved capabilities in the specific detection of bioanalytes for their simple functionalization and high surface volume ratio9. The gold nanoparticles (AuNPs), one of the widely used noble metal nanoparticles, are promising materials for biomedical applications, such as chemical sensing10, photothermal therapy (PTT)11, bio-imaging12, and surface-enhanced raman spectroscopy (SERS)13. In these applications, the size of AuNPs is an important parameter to determine their size-dependent properties14. Uniform-sized nanoparticles can enhance the efficiency of the bios
Data Loading...
