Dual-polarity SALDI FT-ICR MS imaging and Kendrick mass defect data filtering for lipid analysis
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RESEARCH PAPER
Dual-polarity SALDI FT-ICR MS imaging and Kendrick mass defect data filtering for lipid analysis Wendy H. Müller 1 & Alexandre Verdin 1 & Christopher Kune 1 & Johann Far 1 & Edwin De Pauw 1 & Cedric Malherbe 1 & Gauthier Eppe 1 Received: 28 August 2020 / Revised: 30 September 2020 / Accepted: 20 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Lipids are biomolecules of crucial importance involved in critical biological functions. Yet, lipid content determination using mass spectrometry is still challenging due to their rich structural diversity. Preferential ionisation of the different lipid species in the positive or negative polarity is common, especially when using soft ionisation mass spectrometry techniques. Here, we demonstrate the potency of a dual-polarity approach using surface-assisted laser desorption/ionisation coupled to Fourier transform-ion cyclotron resonance (SALDI FT-ICR) mass spectrometry imaging (MSI) combined with Kendrick mass defect data filtering to (i) identify the lipids detected in both polarities from the same tissue section and (ii) show the complementarity of the dual-polarity data, both regarding the lipid coverage and the spatial distributions of the various lipids. For this purpose, we imaged the same mouse brain section in the positive and negative ionisation modes, on alternate pixels, in a SALDI FT-ICR MS imaging approach using gold nanoparticles (AuNPs) as dual-polarity nanosubstrates. Our study demonstrates, for the first time, the feasibility of (i) a dual-polarity SALDI-MSI approach on the same tissue section, (ii) using AuNPs as nanosubstrates combined with a FT-ICR mass analyser and (iii) the Kendrick mass defect data filtering applied to SALDI-MSI data. In particular, we show the complementarity in the lipids detected both in a given ionisation mode and in the two different ionisation modes. Keywords SALDI . Mass spectrometry imaging . Nanoparticles . Dual-polarity . Lipidomics . Kendrick mass defect . FT-ICR
Introduction Lipids are biomolecules of crucial importance and are major structural components of the biological membranes of every living cell [1–3]. These ubiquitous biomolecules are also involved in many critical biological functions such as energy storage, homeostasis and cell signalling [2, 4–6]. Additionally, monitoring the changes in lipid localisation Published in the topical collection Mass Spectrometry Imaging 2.0 with guest editors Shane R. Ellis and Tiffany Siegel Porta. Wendy H. Müller and Alexandre Verdin contributed equally to this work. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00216-02003020-w. * Gauthier Eppe [email protected] 1
Mass Spectrometry Laboratory, MolSys Research Unit, Department of Chemistry, University of Liège, Allée du Six Août, 11 – Quartier Agora, 4000 Liège, Belgium
and expression can help detecting various diseases such as cancer, atherosclerosis or neurodegenerative disorders [4, 5]. Hence, the analysis of
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