Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications
This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling stan
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Ericka A. Pestana · Sandor Belak · Adama Diallo · John R. Crowther · Gerrit J. Viljoen
Early, Rapid and Sensitive Veterinary Molecular Diagnostics - Real Time PCR Applications
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Dr. Ericka A. Pestana Department of Nuclear Sciences and Applications International Atomic Energy Agency (IAEA) A1400 Vienna, Austria [email protected]
Prof. Sandor Belak Swedish University of Agricultural Sciences Department of Virology SE-750 07 Uppsala Sweden [email protected]
Dr. Adama Diallo Department of Nuclear Sciences and Applications International Atomic Energy Agency (IAEA) A1400 Vienna, Austria [email protected]
Dr. John R. Crowther Department of Nuclear Sciences and Applications International Atomic Energy Agency (IAEA) A1400 Vienna, Austria [email protected]
Prof. Gerrit J. Viljoen Department of Nuclear Sciences and Applications International Atomic Energy Agency (IAEA) A1400 Vienna, Austria [email protected]
ISBN 978-90-481-3131-0 e-ISBN 978-90-481-3132-7 DOI 10.1007/978-90-481-3132-7 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009938825 Copyright © International Atomic Energy Agency 2010, Published by Springer Science+Business Media B.V., Dordrecht 2010. All Rights Reserved. No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Foreword
The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, nuclear and nuclear related molecular diagnostic methods have rapidly evolved in the past 20 years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past 5 years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR, Real-Time PCR and PCR-Sequencing as diagnostic and characterization tools in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of
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