Rapid Cycle Real-Time PCR Methods and Applications
Rapid Cycle Real-Time PCR allows quantification and sequence analysis in less than 30 minutes. This powerful technique can be applied to any nucleic acid for rapid testing in microbiology, genetics, and oncology. Fluorescence analysis provides convenient
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S. MEUER
C. WITTWER
K. NAKAGAWARA
Rapid Cycle Real-Time PCR
Springer Berlin Heidelberg New York Barcelona Hong Kong London Milan Paris Singapore Tokyo
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Methods and Applications
With 133 Figures and 209 Tables
Springer
Prof. Dr. med. STEFAN MEUER Ruprecht Karls-Universitat Heidelberg 1nstitut fur 1mmunologie 1m Neuenheimer Feld 305 69120 Heidelberg, Germany Prof. Dr. CARL WITTWER University of Utah Medical School Department of Pathology Salt Lake City, UT 84132, USA Dr. KAN-ICHI NAKAGAWARA Sendai, Japan 2-3-36 Ohgimachi Miyagino-ku Sendai 983-0034, Japan
ISBN-13:978-3-S40-66736-0 Library of Congress applied for Die Deutsche Bibliothek - CIP-Einheitsaufnahme Rapid cycle real time PCR : methods and applications 1 Stefan Meuer ... (ed.). - Berlin; Heidelberg; New York; Barcelona; Budapest; Hong Kong; London; Mailand ; Paris; Santa Clara; Singapore; Tokyo: Springer, 2001 ISBN-13:978-3-540-66736-0 e-ISBN-13: 978-3-642-59524-0 DOl: 10.1007/978-3-642-59524-0 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer- Verlag. Violations are liable for prosecution under the German Copyright Law. Springer- Verlag Berlin Heidelberg New York a member of BertelsmannSpringer Science+ Business Media GmbH ©
Springer-Verlag Berlin Heidelberg 2001
The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher cannot guarantee the accuracy of any information about dosage and application thereof contained in this book. In every individual case the user must check such information by consulting the relevant literature. Cover Design: design & production, 69121 Heidelberg, Germany Production: Pro Edit GmbH, 69126 Heidelberg, Germany Typesetting: TBS, 69207 Sandhausen, Germany 18/3134 Re - 543210 - Printed on acid free paper SPIN 10723838
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Table of Contents
Rapid Cycle Real-Time PCR: Methods and Applications . ................... . CARL WITTWER
Part I Methods Mutation Detection by Fluorescent Hybridization Probe Melting Curves PHILIP S. BERNARD, ASTRID REISER, GREGORY H. PRITHAM
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Quantification on the LightCycier
21
RANDY RASMUSSEN
Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis ............................... . ....... . ......
35
OLFERT LANDT
Using the Nearest Neighbor Model for the Estimation of Matched and Mismat
