Effect of a Multi-Species Probiotic on the Colonisation of Salmonella in Broilers
- PDF / 730,078 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 155 Views
Effect of a Multi-Species Probiotic on the Colonisation of Salmonella in Broilers Deon P. Neveling 1 & L. van Emmenes 2 & J. J. Ahire 1 & E. Pieterse 2 & C. Smith 3 & L. M. T. Dicks 1
# Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Newly hatched broiler chickens are the most susceptible to Salmonella infections, especially during the first 24 h. At this age, the gut microbiome is not fully developed and offers little protection in the form of competitive exclusion. In this study, one group of newly hatched, Salmonella-free broilers were colonised with a multi-species probiotic (2.0 × 1010 to 8.9 × 1010 CFU per kg feed) for 28 days, consisting of Lactobacillus crispatus, Lactobacillus salivarius, Lactobacillus gallinarum, Lactobacillus johnsonii, Enterococcus faecalis and Bacillus amyloliquefaciens. Broilers in another group received oxytetracycline (200 mg/kg feed), instead of the probiotic, for 28 days. On days 9 and 10, broilers in both groups were gavaged with 9 × 107 CFU Salmonella Enteritidis A9, a pathogenic strain isolated from infected broilers. On day 14, Salmonella was detected in the ceca of 95% of broilers treated with the multi-species probiotic, but 2 weeks later, almost half of the birds (45%) had no Salmonella in their ceca. Similar results were recorded after 28 days of treatment with oxytetracycline. Only 10% of Salmonella-infected birds not treated were Salmonella-free on day 28. Growth performance, immune organ weight (spleen and bursa of Fabricius) and whole blood cell counts of birds treated with the multispecies probiotic and oxytetracycline, respectively, were similar to untreated and uninfected birds throughout the 28-day trial (p > 0.05). On day 14, serum lysozyme levels of broilers exposed to the probiotic were lower (8.0 μg/mL) compared with those of broilers treated with oxytetracycline (11.0 μg/mL). Although the multi-species probiotic and oxytetracycline stimulated the immune system, probiotics are safer to use than antibiotics and should be the preferred choice of treatment. Keywords Poultry . Salmonella . Probiotics . Lysozyme blood levels . Immunity
Introduction Serovars of Salmonella enterica subsp. enterica, specifically S. Enteritidis, S. Typhimurium and S. Gallinarum, are responsible for huge financial losses in the poultry industry [1–4]. Despite vast improvements made in housing and feeding, bacterial and fungal infections are still causing substantial financial losses [5]. One of the reasons is that farming has become so Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12602-019-09593-y) contains supplementary material, which is available to authorized users. * L. M. T. Dicks [email protected] 1
Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch 7602, South Africa
2
Department of Animal Science, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch 7602, South Africa
3
Department of Physiological Sciences, University of Stellenbosch, Pri
Data Loading...
