Effect of anisotropic silk fibroin topographies on dorsal root ganglion
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Effect of anisotropic silk fibroin topographies on dorsal root ganglion Yan Kong1, Liling Zhang2,3, Qi Han2,3, Shiyu Chen2,3, Yifan Liu4, Hanshuo Mu4, Yiheng Liu4, Guicai Li2,3,a) , Xiaoyang Chen5,b), Yumin Yang1,2,3,c) 1
Key Laboratory of Eco-Textiles, Ministry of Education, School of Textiles and Clothing, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, Nantong 226001, P.R. China 3 Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, P.R. China 4 School of Medicine, Nantong University, Nantong 226001, P.R. China 5 Department of Ultrasound, Affiliated Hospital of Nantong University, Nantong 226001, P.R. China a) Address all correspondence to these authors. e-mail: [email protected] b) e-mail: [email protected] c) e-mail: [email protected] 2
Received: 22 February 2020; accepted: 13 May 2020
The surface topology of biomaterial has a definite effect on the growth behavior of nerve cells for peripheral nerve regeneration. In this study, the silk fibroin (SF) film with different anisotropic microgroove/ridge was constructed by micropatterning technology. The effects of topologies width on the directional growth of dorsal root ganglion (DRG) neurons were evaluated. The results showed that the topological structure of the SF film with higher SF concentration was more clear and complete. The microtopography of the SF film with a concentration of 15% and a groove width of around 30 μm could effectively guide the directional growth of the nerve fibers of DRG. And nerve fibers could obviously form nerve fiber bundles which may have a certain pavement effect on the recovery of nerve function. The study indicated that the SF film with a specific width of the topological structure may have potential applications in the field of directional nerve regeneration.
Introduction Peripheral nerve injury caused by accidents or surgical side effects has become a global problem, which seriously affects the quality of patients’ life and causes an economic burden [1–3]. Generally, the ability of nerve regeneration and functional recovery is limited due to the weak self-regeneration ability of neurons [4]. For nerve defects with short-gap, the surgical suture techniques were mainly used to connect a divided nerve through end-to-end connection [5, 6], while for nerve defects with long-distance, autologous nerve grafting was still the gold standard. However, the source of the donor nerve was limited [7]. In order to solve the above-mentioned problem, the artificial nerve graft made from various biomaterials has been developed for repairing nerve defects [3, 8]. However, the curative effect of artificial nerve grafts is still not as effective as autologous nerve grafting [9]. Thus, artificial nerve graft with better performance for promoting nerve regeneration needs further development. Artificial nerve grafts need to simulate natural nerve not only in composition but also in a three-dimensional (3D)
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