Effect of Anti-Nematode Preparations on Physiological Traits of Cucumber Leaves Affected by Root-Knot Nematode

This paper was to study the effects of anti-nematode preparations with different mechanisms on changes of enzyme systems and membrane permeability of cucumber leaves, so as to provide reference basis for effective control of soil root-knot nematode in gre

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Effect of Anti-Nematode Preparations on Physiological Traits of Cucumber Leaves Affected by Root-Knot Nematode Shuchang Lu

Abstract This paper was to study the effects of anti-nematode preparations with different mechanisms on changes of enzyme systems and membrane permeability of cucumber leaves, so as to provide reference basis for effective control of soil root-knot nematode in greenhouse. With cucumber seedlings affected by root-knot nematode as material, changes of superoxide dismutase (SOD), peroxidase (POD), relative conductivity in cucumber were tested after the seedling soil was treated by preparations of Wuxianmei, Hailvsu, Duxiandna, and Avermectin. After treated by different preparations, SOD and POD activity of cucumber leaves were higher than control, and that treated by Wuxianmei was the highest. In addition to Duxiandna, the relative conductivity content of other treatments were significantly lower than control. When cucumbers were planted for 70 days, the effect of Avermectin against root-knot nematode was the best of 64.7 %. After cucumbers were infected by root-knot nematode, different preparation treatments all had certain control effect, which made the physical indicators of cucumber have obvious change.









Keywords Cucumber Root-knot nematode Preparation Leaf Physiological trait

104.1 Introduction The damage of soil root-knot nematode in the main vegetables producing areas is increasingly serious in China, and it shows significant aggravation trend especially in northern producing area of off-season protected vegetables, being a serious obstacle restricting the production of protected vegetables. S. Lu (&) Department of Agronomy, Tianjin Agricultural University, 300384 Tianjin, People’s Republic of China e-mail: [email protected]

T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_104, Ó Springer-Verlag Berlin Heidelberg 2014

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According to the survey, the disease has different degrees of occurrence in the main producing areas in Jixian, Wuqing, and Jinghai of Tianjin City, especially in Jixian, root-knot nematode disease has caused great harm on the local vegetable production [1]. When root-knot nematode infects the roots of cucumber, the enzyme systems and membrane permeability of root system will change, thus affecting the physiological traits of aboveground leaf tissue. This not only directly affects the growth and development of vegetables, causing the production decline and quality reduce, but also exacerbates the occurrence of bacterial and fungal diseases such as Fusarium wilt [2]. Currently, the mechanisms for the prevention of soil root-knot nematodes are as follows: (1) using preparations to kill root-knot nematode; (2) using preparations to drive root-knot nematodes away from the roots to prevent roots being infected; (3) using root growth promoting preparation to promote the growth of new roots, thus increasing the tr