Effect of pH on the activity of ice-binding protein from Marinomonas primoryensis
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ORIGINAL PAPER
Effect of pH on the activity of ice‑binding protein from Marinomonas primoryensis Elizabeth A. Delesky1 · Patrick E. Thomas2,3 · Marimikel Charrier4 · Jeffrey C. Cameron2,3,5 · Wil V. Srubar III1,4 Received: 19 June 2020 / Accepted: 29 September 2020 © Springer Japan KK, part of Springer Nature 2020
Abstract The ability of an ice-binding protein (IBP) from Marinomonas primoryensis (MpIBP) to influence ice crystal growth and structure in nonphysiological pH environments was investigated in this work. The ability for MpIBP to retain ice interactivity under stressed environmental conditions was determined via (1) a modified splat assay to determine ice recrystallization inhibition (IRI) of polycrystalline ice and (2) nanoliter osmometry to evaluate the ability of MpIBP to dynamically shape the morphology of a single ice crystal. Circular dichroism (CD) was used to relate the IRI and DIS activity of MpIBP to secondary structure. The results illustrate that MpIBP secondary structure was stable between pH 6 and pH 10. It was found that MpIBP did not interact with ice at pH ≤ 4 or pH ≥ 13. At 6 ≤ pH ≥ 12 MpIBP exhibited a reduction in grain size of ice crystals as compared to control solutions and demonstrated dynamic ice shaping at 6 ≤ pH ≥ 10. The results substantiate that MpIBP retains some secondary structure and function in non-neutral pH environments; thereby, enabling its potential utility in nonphysiological materials science and engineering applications. Keywords Ice-binding proteins · Antifreeze proteins · pH · Ice recrystallization inhibition · Dynamic ice shaping
Introduction Previous research indicates that ice-binding proteins (IBPs) may offer an alternative to conventional frost–prevention strategies for biological cryopreservation (Davies 2014; Liang et al. 2016) and, by extension, antifreeze applications in a host of other commercial industries such as coolants in aerospace engineering, frost–resistant pavements in civil Communicated by A. Driessen. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00792-020-01206-9) contains supplementary material, which is available to authorized users.
engineering, and anti-icing coatings for energy infrastructure such as solar panels or wind turbines. While IBPs offer a promising biological solution for these ice-growth prevention applications, proteins are well known to unfold, refold, denature, aggregate, or degrade in nonphysiological environments (Ptitsyn 1987). Applications with harsh chemical environments, such as concrete in civil engineering that has a pore solution pH of 12–13 (Ghods et al. 2009), would benefit from a material that inhibits ice recrystallization. Freeze–thaw damage in concrete is due, in part, to the expansion of ice crystals (Powers 1975) demonstrating a need for materials that inhibit ice growth in extreme pH environments. To the authors’ knowledge, some studies have
* Wil V. Srubar III [email protected]
1
Materials Science and Engineering Program, University of
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