Research on the Optimum Conditions and the Effect of Antioxidant Peptide from Scallop Protein
Bay scallop and Japanese scallop were chosen for enzymatic hydrolysis by neutral protease, alkaline protease, and papain, and the enzymolysis technology of scallop protein was optimized to obtain antioxidant peptide. The effect of kinds of scallop and enz
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Research on the Optimum Conditions and the Effect of Antioxidant Peptide from Scallop Protein Yuan Liu, Jian Wang, Zhisheng Zhang and Jie Wang
Abstract Bay scallop and Japanese scallop were chosen for enzymatic hydrolysis by neutral protease, alkaline protease, and papain, and the enzymolysis technology of scallop protein was optimized to obtain antioxidant peptide. The effect of kinds of scallop and enzyme, enzyme quantity, temperature, and the ratio of solid to liquid on the degree of hydrolysis (DH) and the free radical scavenging activity was studied. On the basis of single factor experiment, orthogonal experiment of L9(34) was used to optimize the technical parameters. The results indicated that the optimal hydrolytic conditions were as follows: using Bay scallop as the material, the enzyme quantity of the Neutral Protease was 3.25 % ([E]/[S]), temperature was 40 °C, and the ratio of solid to liquid was 1:2.5. Under this condition, the DH was 45.91 %, and the scavenging rate of DPPH radical and superoxide anion radical were 91.90 % and 79.72 % respectively. Keywords DPPH radical
Antioxidant peptide Scallop Superoxide anion
165.1 Introduction Because of the shortage of terrestrial resources, much attention is increasingly paid to find the novel active material from marine organism. Therefore, more and more active material has been gradually found from marine shellfish. Polypeptide from chlamys farreri (PCF) is a water-soluble polypeptide isolated first from chlamys Y. Liu Z. Zhang J. Wang (&) College of Food Science and Technology, Agriculture University of Hebei, Baoding 071000, People’s Republic of China e-mail: [email protected] Y. Liu J. Wang Department of Food Science, Hebei North University, Zhangjiakou 075000, People’s Republic of China
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 251, DOI: 10.1007/978-3-642-37925-3_165, Ó Springer-Verlag Berlin Heidelberg 2014
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farreri. The study of cultured cells has found that PCF can efficiently remove oxygen free radicals produced by chemical systems [1], inhibit the oxidative stress of UVA on Hela epithelial cells [2], the oxidative stress of UVB on fibroblasts [3], obviously relieve the damage effects of UVA and UVB on the skin of km mutant hairless mice [4], increase the immunologic cellular activity, relieve the oxidative stress of thymocyte, inhibit thymocyte apoptosis [5], and avoid the immune cells damaged by ultraviolet and (60)Co radiation, and so on [6, 7]. So PCF, as a new natural antioxidant, is of great exploitation value. Presently, many kinds of polypeptides with antioxidant activity have been extracted from different proteins [8, 9]. In this research, Scallop was chosen for enzymatic hydrolysis by protease to obtain antioxidant peptide. On the basis of screening experiment and single factor experiment, the technical parameters were optimized by orthogonal experiment, and the antioxidant obtained was u
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