Effect of Tween Type Non-Ionic Detergent on the Activity of Lipase of Pseudomonas aeruginosa

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ORIGINAL PAPER

Effect of Tween Type Non-Ionic Detergent on the Activity of Lipase of Pseudomonas aeruginosa Maryam Shoja1 Dariush Minai-Tehrani ●

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Accepted: 21 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020

Abstract Pseudomonas aeruginosa is a Gram-negative and rod-shaped bacterium. It can use a variety of carbon sources and grow in different culture media. Its versatile extracellular enzymes give it the ability to grow on complex carbon sources. One of the most important enzymes of this bacterium is lipase, which is an extracellular enzyme. Lipases are one of the most useful enzymes in medicine and industry, especially in the detergent industry. In recent years, lipases have become an important component of detergent powders, so it is important to evaluate the performance of lipases in the presence of detergents. The aim of this study was to investigate the effect of non-ionic detergents Tween 20 and 80 on the activity of the Pseudomonas lipase. These detergents reduced Km and increased Vmax of the enzyme. The enzyme activity increased in the presence of these detergents at optimal pH and temperature. Conformational studies with the purified enzyme by fluorescence spectrophotometry showed that in the presence of Tween 20 and 80, there was a hypochromicity in emission peak of the enzyme, which indicated that the enzyme became less compact in vicinity of these detergents. Keywords Lipase Bacteria Detergent Activity Pseudomonas Tween ●







Introduction Pseudomonas aeruginosa is a Gram-negative bacillus that is often found in soil and groundwater and is an opportunistic pathogen [1]. This bacterium rarely causes disease in healthy people. The disease usually occurs in patients whose immune systems are weakened by another underlying health problem [2, 3]. The bacterium also has useful applications in various industrial and commercial sectors, including waste removal, oil biodegradation, textile products, agriculture, pulp and paper pulp, mining industries [4–6]. P. aeruginosa can live in a wide range of environmental conditions, it is known as a “ubiquitous” microorganism. This versatility is made possible by a large number of enzymes that allow it to use a variety of substances as nutrients. It is an effective producer of extracellular enzymes such as protease, lipase and nuclease which can be

* Dariush Minai-Tehrani [email protected] 1

BioResearch Lab, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran



used in industry. Recent researches show that P. aeruginosa is one of the best producers of lipase, which has received much attention due to the use of this enzyme in various industries [7–9]. Lipase is known as an important component in household detergents [10], where their function is to help remove lipid residues. Many washing powder manufacturers use lipase in their detergent formulations [11, 12]. Because fats and oils are hydrophobic, it is difficult to wash and remove them from clothing and