The role of Pseudomonas aeruginosa DesB in pathogen-host interaction
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ORIGINAL ARTICLE
The role of Pseudomonas aeruginosa DesB in pathogen-host interaction Jimyeong Ha 1 & Sejeong Kim 1 & Jeeyeon Lee 2 & Heeyoung Lee 3 & Yukyung Choi 4 & Hyemin Oh 4 & Yohan Yoon 1,4 & Kyoung-Hee Choi 5 Received: 13 December 2019 / Revised: 19 March 2020 / Accepted: 13 April 2020 # Springer Nature Switzerland AG 2020
Abstract Pseudomonas aeruginosa, commonly found in environments, can cause chronic lung disease in immunocompromised patients. In previous study, an aerobic desaturase (DesB) in P. aeruginosa exerted considerable effects on virulence factor production. The objective of this study was to analyze the role of DesB on the virulence traits of P. aeruginosa in the host. For the in vitro experiments, cells and supernatants from wild-type (WT) P. aeruginosa and its desB mutant were collected. The diluted cells were added to the A549 cell monolayer in order to determine cell viability, invasion ability, and/or immune response. For the in vivo experiments, 6-week-old ICR mice were infected with 6–7 log CFU bacterial cells using endotracheal intubation. The ratio of lung weight to body weight and survival rate of each bacterial strain in the lung were measured. The histopathology of lung tissue was also studied. desB mutants exhibited lower cytotoxicity in A549 cells. In addition, more pro-inflammatory cytokines and chemokines were present in desB mutant-treated. In the lungs of mouse model, WT survived longer than desB mutant, and the WT migrated from the lung to the liver and spleen. The results suggest that P. aeruginosa DesB affects the pathogenicity of the organism in the host. Keywords Pseudomonas aeruginosa . DesB . Lung disease . Pathogenicity
Introduction Pseudomonas aeruginosa is an opportunistic gram-negative bacterium that exists in diverse environments such as soil, plants, and animal tissue (Hardalo and Edberg 1997). It is a pathogen that causes harmful chronic lung damage in patients Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10123-020-00130-4) contains supplementary material, which is available to authorized users. * Kyoung-Hee Choi [email protected] 1
Risk Analysis Research Center, Sookmyung Women’s University, Seoul 04310, South Korea
2
Department of Food and Nutrition, Dong-eui University, Busan 47340, South Korea
3
Food Standard Research Center, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, South Korea
4
Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, South Korea
5
Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan 54538, South Korea
with cystic fibrosis as well as in immunocompromised patients (Rieber et al. 2013). The pathogen has a variety of virulence characteristics, such as the type III secretion, quorumsensing systems, endotoxins, and exotoxins. These virulence factors contribute towards the activation of host defense and inflammatory responses (Kipnis et al. 2006). Surface factors of P. aeruginosa, in particular, play a critical ro
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