Effective serological and molecular screening of deceased tissue donors
- PDF / 213,525 Bytes
- 12 Pages / 547.087 x 737.008 pts Page_size
- 77 Downloads / 186 Views
ORIGINAL PAPER
Effective serological and molecular screening of deceased tissue donors A. D. Kitchen • J. A. Newham • H. L. Gillan
Received: 2 October 2012 / Accepted: 17 December 2012 / Published online: 29 January 2013 Ó Crown Copyright as represented by the NHS Blood and Transplant 2013
Abstract A comprehensive and effective screening programme is essential to support the banking of tissues from deceased donors. However, the overall quality of the samples obtained from deceased donors, quantity and condition, is often not ideal, and this may lead to problems in achieving accurate and reliable results. Additionally a significant percentage of referrals are still rejected upon receipt as unsuitable for screening. We are actively involved in improving the overall quality of deceased donor screening outcomes, and have specifically evaluated and validated both serological and molecular assays for this purpose, as well as developing a specific screening strategy to minimise the specificity issues associated with serological screening. Here we review the nature and effectiveness of the deceased donor screening programme implemented by National Health Service Blood and Transplant (NHSBT), the organisation with overall responsibility for the supply of tissue products within England. Deceased donor screening data, serological and molecular, from August 2007 until May 2012 have been collated and analysed. Of 10,225 A. D. Kitchen (&) J. A. Newham National Transfusion Microbiology Reference Laboratory, NHS Blood and Transplant, Charcot Road, Colindale Avenue, London NW9 5BG, UK e-mail: [email protected] H. L. Gillan Tissue Services, NHS Blood and Transplant, Estuary Banks, Speke, Liverpool L24 8RB, UK
samples referred for serology screening, 5.5 % were reported as reactive; of 2,862 samples referred for molecular screening, 0.1 % were reported as reactive/ inhibitory. Overall 20 % of the serological and 100 % of the molecular screen reactivity was confirmed as reflecting true infection. The use of a sequential serology screening algorithm has resulted in a marked reduction of tissues lost unnecessarily due to nonspecific screen reactivity. The approach taken by NHSBT has resulted in the development of an effective and specific approach to the screening of deceased tissue donors. Keywords Deceased donor Tissue donor Serological screening Molecular screening Organ donor
Introduction The screening of deceased tissue donors is a critical activity to ensure the safety and availability of the wide range of tissue products required by medicine today. Prior to release for clinical use tissue donations retrieved from deceased donors require laboratory screening for any evidence of the presence of a range of infectious agents that may be transmitted via tissue transplantation. In the UK this is performed on a venous blood sample collected from the donor up to 1 week pre-mortem or, and more usually in our experience, up to 24 h post-mortem.
123
634
Screening is performed for the ‘standard set’ of transmissible
Data Loading...
