Effects of heavy metals on the expression of digestive enzyme-coding genes in the brackish water flea Diaphanosoma celeb
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ORIGINAL ARTICLE
Effects of heavy metals on the expression of digestive enzyme‑coding genes in the brackish water flea Diaphanosoma celebensis Jiyoon Lee1 · Young‑Mi Lee1 Accepted: 30 September 2020 © Korean Society of Environmental Risk Assessment and Health Science 2020
Abstract Objectives Energy metabolism is an essential physiological event for all life forms, and, in heterotrophic organisms, it significantly depends on food intake. Digestive enzymes play an important role in acquiring the nutrients required for metabolic pathways. Thus, the aim of this study was to investigate the transcriptional modulation of digestive enzyme-coding genes in response to heavy metals in the brackish water flea Diaphanosoma celebensis. Methods Sublethal concentrations of inorganic arsenic (As), cadmium (Cd), and lead (Pb) on the brackish water flea D. celebensis were calculated from the 48 h acute toxicity test result. The relative mRNA expression levels of six digestive enzyme-coding genes (AMY and AMY-like for carbohydrates; TRYP and CHY for proteins; LIP and PLRP for lipids) after 48 h of exposure to heavy metals were evaluated by real-time reverse transcription-polymerase chain reaction. Results A significant increase in the mRNA expression of AMY, AMY-like, TRYP, and PLRP was observed in all concentration of As (1.17–3.94 fold-change, p
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