Effect of the suppression of BpAP1 on the expression of lignin related genes in birch
- PDF / 3,388,297 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 91 Downloads / 184 Views
ORIGINAL PAPER
Effect of the suppression of BpAP1 on the expression of lignin related genes in birch Haijiao Huang1 · Shuo Wang1 · Huiyu Li1 · Jing Jiang1
Received: 15 June 2020 / Accepted: 17 July 2020 © Northeast Forestry University 2020
Abstract Lignin is an integral part of secondary cell walls in plants and plays important roles in maintaining the strength of stems, enhancing transport ability of stems, and providing resistance to multiple stresses. Lignin biosynthesis has become one of the hotspots in molecular forest biology research. The AP1 transcription factor plays important roles in plant flower development. However, in this study, suppression of BpAP1 altered the transcription profiles of white birch and RNA-seq was used to find that suppression of BpAP1 changed the expression of lignin pathway-related genes; C4H/CYP73A, POD were down-regulated and HCT, CCoAOMT, REF1 and CAD were up-regulated. Cell walls of the suppressed transgenic birch were significantly thinner than the wild type of birch, and BpAP1- repressed birch contained less lignin. In addition to regulation of floral development, BpAP1 might play a role in regulating the expression of genes in lignin biosynthesis of birch. This study could
Project funding: This study was supported by the National Natural Science Foundation of China (31800556), and the Fundamental Research Funds for the Central Universities (2572018BW06). The online version is available at https://www.springerlink.com. Corresponding editor: Yu Lei. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11676-020-01232-5) contains supplementary material, which is available to authorized users. * Jing Jiang [email protected] 1
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, People’s Republic of China
provide a new insight into the function of AP1 genes in woody species. Keywords BpAP1 · Lignin biosynthesis · RNA-seq · Wood fiber cell walls
Introduction Transcription factor (TF), also known as trans-acting factors, directly or indirectly interact with cis-acting elements in the promoter regions of downstream genes, a group of proteins that regulate the initiation of gene transcription. Transcription factors have been described figuratively as “switches that control several lights” at once. Their expression activates or inhibits the expression of a series of genes. The transcription translation product of APETALA1 (AP1) gene is a transcription factor in plants. Several studies have reported that over expression of the AP1 gene can accelerate flowering time and significantly shorten the juvenile phase (Irish and Sussex 1990; Mandel et al. 1992; Elo et al. 2001; Pea et al. 2001; Cseke et al. 2003; Fernando and Zhang 2005; Kotoda et al. 2005; Jaya et al. 2009; Chi et al. 2011; Huang et al. 2014). Published research of AP1 mainly focuses on its canonical function in regulating flower development, and a previous study by Huang et al. (2014) found that the BpAP1 in Betula
Data Loading...
