Effects of hydroxyapatite-coated nonwoven polyethylene/polypropylene fabric on non-mesodermal lineage-specific different
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BMC Research Notes Open Access
RESEARCH NOTE
Effects of hydroxyapatite‑coated nonwoven polyethylene/polypropylene fabric on non‑mesodermal lineage‑specific differentiation of human adipose‑derived stem cells Edward Hosea Ntege1, Hiroshi Sunami2, Junko Denda2, Naoko Futenma1 and Yusuke Shimizu1*
Abstract Objective: Compared to other stem cells, the multipotency of human adipose-derived mesenchymal stem cells (ASCs) is limited. Effective approaches that trigger or enhance lineage-specific transdifferentiation are highly envisaged in the improvement of ASCs-based cell therapies. Using Immunofluorescence assays and the secretion of cardiac troponin T (cTnT) protein, we studied the impact of two substrates: Hydroxyapatite (HAp)-coated nonwoven polyethylene (PET)/ polypropylene (PP) fabric and glass surfaces, representing 3 dimensional (D) and 2 D environments respectively, on the induction of cardiomyocytes – a non-mesodermal cell type from ASCs for 1–5 weeks. Results: ASCs were successfully isolated from human adipose tissue under cGMP conditions. Within 1–3 weeks, expression of cTnT in the induced 3D cultures was overall significantly higher (P
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