Effects of N application on N accumulation of two Erigeron breviscapus populations from different altitudes under enhanc
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ORIGINAL PAPER
Effects of N application on N accumulation of two Erigeron breviscapus populations from different altitudes under enhanced ultraviolet-B radiation Yuan Li • Yanqun Zu • Jing Ji • Yongmei He
Received: 28 May 2013 / Revised: 2 July 2013 / Accepted: 27 August 2013 / Published online: 8 September 2013 Ó Franciszek Go´rski Institute of Plant Physiology, Polish Academy of Sciences, Krako´w 2013
Abstract To study intraspecific differences in N utilization in response to enhanced UV-B radiation, field experiments were conducted on two Erigeron breviscapus populations (Huguo and Cangshan), which were respectively obtained from low altitude (UV-B sensitive) and high altitude (UBV-B resistant).The effects of soil nitrogen (N) application (0, 15, 30, 45 g m2) on free amino acid content, the activities of nitrate reductase (NR) and glutamine synthetase (GS), total nitrogen content and N mass in leaves were determined under enhanced UV-B radiation (5 kJ m2) for both populations. The results showed that under enhanced UV-B radiation: (1) increases in total N contents in leaves of the Huguo and Cangshan populations correlated with the amount of N applied. Additionally, leaf biomass of Huguo treated with 15 g m-2 N application and Cangshan with 30 g m-2 N application were higher than that of other treatments. Leaf N masses were highest in both E. breviscapus populations treated with 30 g m-2 N; (2) increases in contents of free amino acids in leaves of both E. breviscapus populations correlated with the amount of applied nitrogen; (3) increases of NR activity in leaves correlated with the amount of applied nitrogen; (4) GS activity in leaves of the Huguo and Cangshan E. breviscapus populations were highest with respective N applications of 15 g m-2 N and 30 g m-2 N. In general, under enhanced UV-B radiation, N application might affect NR and GS and change free amino acid content, resulting in changes in total nitrogen content, biomass and N mass. The
Communicated by L. A. Kleczkowski. Y. Li Y. Zu (&) J. Ji Y. He College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, People’s Republic of China e-mail: [email protected]
optimal amount of supplemental N for N accumulation in E. breviscapus was 30 g m-2 N under enhanced UV-B radiation. Keywords Erigeron breviscapus UV-B radiation N application N accumulation Nitrate reductase (NR) Glutamine synthetase (GS)
Introduction Ambient levels of UV-B radiation (k = 280–315 nm) in the atmosphere have risen significantly over the past few decades due to a decrease in stratospheric ozone (Caldwell et al. 2007). Many studies about the impact of enhanced UV-B radiation on plants have demonstrated that altered levels of exposure to UV-B radiation can have a significant effect on plant growth, morphology, physiology and biomass production (Kadur et al. 2007; Qaderi et al. 2008; Roblek et al. 2008; Calderini et al. 2008; Liu et al. 2011). Nitrogen metabolism is one of basic physiological processes, which includes conversion of nitrate into
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