Evaluation of biochemical parameters in Rubus tereticaulis treated rats and its implications in wound healing
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Evaluation of biochemical parameters in Rubus tereticaulis treated rats and its implications in wound healing Halil Aksoy1 · Çağlar Demirbağ2 · Ali Şen3 · Turgut Şekerler1 · Özlem Özakpınar1 · Azize Şener1 · Sarfraz Ahmad4 · Sermin Tetik1 Received: 9 April 2020 / Accepted: 4 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract We evaluated the effects of Rubus tereticaulis in healing process by determining the total carbonyl content, collagen synthesis, and total protein level on rat wounded tissues. Wounds were performed in the back of 54 Wistar rats, using a biopsy punch instrument with 0.6 mm in diameter. Rats were randomly divided into three groups: (i) un-treatment wounds group served as “controls”, (ii) Madecassol® used as “positive control” group, and (iii) the application of topical cream of R. tereticaulis served as “treatment” group of wound healing. The animals were killed at the end of experiment under anesthesia with ketamine, and tissue samples were collected for the evaluation at three times intervals (3rd, 7th, and 14th day). The wounded areas were analyzed for total carbonyl content, collagen, and total protein levels by HPLC, ELISA, and spectrophotometric methods, respectively. Total carbonyl content in the treatment group was significantly lower in comparison with control group on 3rd day (2.839 ± 0.438 vs. 3.216 ± 0.216 nmol carbonyl/mol protein; p
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