Effects of SDF-1/CXCR4 on the Repair of Traumatic Brain Injury in Rats by Mediating Bone Marrow Derived Mesenchymal Stem
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ORIGINAL RESEARCH
Effects of SDF-1/CXCR4 on the Repair of Traumatic Brain Injury in Rats by Mediating Bone Marrow Derived Mesenchymal Stem Cells Quan-Jun Deng1 • Xiao-Feng Xu2,3 • Jing Ren4
Received: 29 December 2016 / Accepted: 24 March 2017 Ó Springer Science+Business Media New York 2017
Abstract Our study aims to investigate the effects of the SDF-1/CXCR4 axis on the repair of traumatic brain injury (TBI) in rats by mediating bone marrow derived from mesenchymal stem cells (BMSCs). Healthy male SD rats were collected, their tibiofibulars were removed, cultured, and BMSCs were collected. The expression of cell-surface molecular proteins was examined using flow cytometry. The mRNA and protein expression of CXCR4 in cells were tested using qRT-PCR and western blotting analysis. An electronic brain injury instrument was utilized to build TBI rat models and each rat was assigned into the experiment, positive control and control groups (10 rats in each group). The morris water maze was used to calculate the escape latency and number of times rats in each group crossed the platform. Neurological severity scores (NSS) was calculated to evaluate the recovery of neurological functioning. The distribution of neuronal nuclear antigens was detected using double-labeling
immunohistochemistry. The morphological changes in the hippocampal neuronal and the number of BrdU-positive cells were observed through Nissl’s staining and high magnification. The mRNA and protein expressions of CXCR4 were gradually increased as SDF-1 concentration increased. NGF and BDNF positive cells were expressed in each group. The distribution of neuronal nuclear antigens in the experiment group was elevated compared to the control and positive control groups. Among the three groups, the experimental group had the shortest escape latency and the highest number platform crossings. The difference in NSS among the three groups was significant. The experimental group had better cell morphology and a higher number of BrdU-positive cells than the other groups. The present study demonstrates that transplanting BMSCs with SDF-1-induced CXCR4 expression can promote the repair of TBI. This is expected to become a new treatment regimen for TBI. Keywords SDF-1 CXCR4 Traumatic brain injury Bone mesenchymal stem cells and cell transplantation
& Jing Ren [email protected] 1
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, People’s Republic of China
2
Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai 200032, People’s Republic of China
3
Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200032, People’s Republic of China
4
Department of Medical Laboratory, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, People’s Republic of China
Abbreviations TBI Traumatic brain injury NGF Nerve growth factor BDNF Brain-derived neurotrophic factor SDF-l Stromal cell-derived factor-l CXCR4 Chemotaxis cytokine rece
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