Biological properties of bone marrow stem cells and adipose-derived stem cells derived from T2DM rats: a comparative stu
- PDF / 7,078,261 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 39 Downloads / 176 Views
(2020) 10:102 Wang et al. Cell Biosci https://doi.org/10.1186/s13578-020-00465-5
Open Access
RESEARCH
Biological properties of bone marrow stem cells and adipose‑derived stem cells derived from T2DM rats: a comparative study Lei Wang1,2*, Shaojie Shi3, Ruiping Bai4, Yue Wang1,2, Zhao Guo1,2 and Doudou Li3
Abstract Background: Patients with type 2 diabetes mellitus (T2DM), especially those with poor glycemic control, are characterized by low bone mass and destruction of bone microstructure. Nowadays, autologous mesenchymal stem cells (auto-MSCs) have been used to repair defects and promote tissue regeneration due to handy source, low immunogenicity and self-renewing and multi-differentiating potential. However, T2DM changed the biological properties of auto-MSCs, and investigating the most suitable auto-MSCs for T2DM patients becomes a focus in tissue engineering. Results: In this research, we compared the biological characteristics of adipose-derived stem cells (ASCs) and bone marrow stem cells (BMSCs) derived from T2DM rats. These results demonstrated that ASCs had a higher proliferation rate, colony-formation and cell-sheet forming ability, while BMSCs got better osteogenesis-related staining, expression of osteogenesis-related genes and proteins, and osteogenic capacity in vitro. Conclusions: As it turned out, ASCs from T2DM had a higher proliferation, while BMSCs had significantly higher osteogenetic ability no matter in vitro and in vivo. Therefore, we should take into account the specific and dominated properties of MSC according to different needs to optimize the protocols and improve clinical outcomes for tissue regeneration of T2DM patients. Keywords: Type 2 diabetes mellitus, Mesenchymal stem cells, Cell proliferation, Osteogenic induction, Cell sheets Introduction Diabetes mellitus, characterized by high glucose levels, is a chronic metabolic disorder, the incidence of which ranks second only after cardiovascular disease. It could be categorized into type 1 diabetes mellitus (T1DM) and T2DM, of which T2DM accounts for 90–95% [1]. Diabetic osteoporosis, characterized with low bone mass and destruction of bone microstructure, is adverse for diabetes patients due to hyperglycemic toxicity, abnormal insulin and cytokine levels as well as oxidative stress [2, 3]. The fracture risk increased and the *Correspondence: [email protected] 1 Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, 98 XiWu Road, Xi’an 710004, Shaanxi, People’s Republic of China Full list of author information is available at the end of the article
fracture healing decreased significantly at the same time in diabetic patients [4]. The proliferation and osteogenesis of alveolar bone-derived osteoblasts from T2DM were impaired [5]. Patients with poor glycemic control had inferior osseointegration of dental implants [6, 7], compromised wound healing process in the early stage [8]. In recent years, the discovery of stem cells has prompted a number of
Data Loading...
