EgPHI - 1 , a PHOSPHATE - INDUCED - 1 gene from Eucalyptus globulus , is involved in shoot growth, xylem fiber length an
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ORIGINAL ARTICLE
EgPHI‑1, a PHOSPHATE‑INDUCED‑1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties Aurizangela O. Sousa1 · Luciana R. Camillo2 · Elza Thaynara C. M. Assis2 · Nathália S. Lima2 · Genilson O. Silva2 · Rochele P. Kirch3 · Delmira C. Silva2 · André Ferraz4 · Giancarlo Pasquali3 · Marcio G. C. Costa2 Received: 30 May 2020 / Accepted: 27 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion EgPHI-1 is a member of PHI-1/EXO/EXL protein family. Itsoverexpression in tobacco resulted in changes in biomass partitioning, xylem fiberlength, secondary cell wall thickening and composition, and lignification. Abstract Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits. Our results indicated that this gene is a member of the PHI-1/EXO/EXL family. Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought. EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots. The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness. UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types. Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops. Keywords Cellulose · Exordium · Lignin · Secondary cell wall · Wood quality Communicated by Dorothea Bartels. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03450-x) contains supplementary material, which is available to authorized users. * Marcio G. C. Costa [email protected]; [email protected] 1
Centro Multidisciplinar do Campus de Luís Eduardo Magalhães, Universidade Federal do Oeste da Bahia, Luís Eduardo Magalhães, Bahia 47850‑000, Brazil
2
Centro de Biotecnologia e Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Bahia 45662‑900, Brazil
3
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91501‑970, Brazil
4
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo- USP, Lorena, São Paulo 12602‑810, Brazil
Abbreviations EXO EXORDIUM EXL EXORDIUM-Like SCW Secondary cell wall PHI-1 PHOSPHATE-INDUCED PROTEIN 1 EgPHI-1
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