Electrochemical detection of non-labeling DNA using electronic array
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Electrochemical detection of non-labeling DNA using electronic array
H.Y.Lee, J.W.Park, Y.S.Choi, T.Kannno, Hiro.Tanaka, Hide.Tanaka, and T.Kawai a) The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 µM concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.
a) Corresponding author: Electronic mail: [email protected]
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I. Introduction Technology of DNA chip has been moving very rapidly in the biosensor field and will in all possibility continue to do so in the near future. From an academic and industrial point of view, DNA array methods are part of the more general biochip scene, in which extreme miniaturization of the analytical sensors is found to adapt the millions of assays. Until now, reported typical protocols for detecting small quantities verified hybridization with fluorescence or chemiluminescences. Because, those detection methods usually require expensive reagents and large detection equipment, the developments of electrochemical biosensors for rapid and high sensitive DNA hybridization with compact detection equipment is required for real application. The main characteristic of our system is the electrochemical approaches include the detection of hybridization from nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In particular, the mechanism of biosensors have been developed until now is the detection of hybridization of unlabeled DNA using optical methods, quartz-crystal microbalance and surface-plasmon resonance. Because of the recent success of electrochemical blood-glucose monitoring, there is considerable enthusiasm for electrochemical detection and monitoring methods throughout the biotechnology industry. The most common electrochemical strategies for detecting the hybridization of unlabeled DNA rely on redox-active hybridization indicators that bind more strongly to DNA duplexes than to single-stranded DNA. Until now, a reported typical protocol for detecting small quantities verified hybridization with fluorescence or chemiluminescence’s. The detection method usually requires expensive reagents and detection equipment. In order to generate bio-functional surfaces, the avidin-biotin system is frequently
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used. The avidin (Av)-biotin technology is a universal molecular system in the biological science. The use of the egg white protein avidin is often restricted due to its high isoelectric point and
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