Gold nanoparticle/DNA-based nanobioconjugate for electrochemical detection of Zika virus
- PDF / 1,626,383 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 12 Downloads / 204 Views
ORIGINAL PAPER
Gold nanoparticle/DNA-based nanobioconjugate for electrochemical detection of Zika virus Sebastian Cajigas 1 & Daniel Alzate 1 & Jahir Orozco 1 Received: 24 June 2020 / Accepted: 25 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The development of a stable nanobioconjugate based on gold nanoparticles (AuNPs) linked to single-strand DNA (ssDNA) is reported for amplification of the electrochemical signal of a Zika virus (ZIKV) genetic material-based bioassay, with high sensitivity. The genosensor was assembled either at a screen-printed gold electrode (SPAuE) or a screen-printed carbon electrode decorated with hierarchical gold nanostructures (SPCE/Au), with Ru3+ as an electrochemical reporter. The genosensor response, interrogated by differential pulse voltammetry (DPV) at the transient current density, was linear from 10 to 600 fM and from 500 fM to 10 pM of the target, with a sensitivity of 2.7 and 2.9 μA cm−2 M−1 and a limit of detection of 0.2 and 33 fM at the SPAuE and SPCE/Au, respectively. The resultant genosensor detected ZIKV genetic material in raw serum samples from infected patients, with no sample pretreatment in a polymerase chain reaction amplification-free assay. The proposed ultrasensitive nanobioconjugate-based system offers a step forward to the diagnosis of the ZIKV, closer to the patient, and holds the potential for signal amplification in biosensing of a myriad of applications. Keywords Nanobioconjugate . Gold nanoparticle . DNA . RNA . Zika virus . Genoassay
Introduction Bioassays for the detection of pathology-associated biomolecules are limited by the low levels in which biomarkers are present in clinical samples, thus hindering diagnostic. For example, the current diagnosis of ZIKV includes the detection of low copies of viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR) and serological detection of low concentrations of anti-ZIKV antibodies. Whereas RT-PCR has high requirements of equipment and well-trained personnel, in the serologic method, antibodies from other homolog arboviruses such as dengue (DENV) and chikungunya (CHIKV) may cross-react [1], thus hindering accurate and timely diagnostic. To circumvent such
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04568-1) contains supplementary material, which is available to authorized users. * Jahir Orozco [email protected] 1
Max Planck Tandem Group in Nanobioengineering, University of Antioquia, Complejo Ruta N, Calle 67 N° 52–20, Medellín 050010, Colombia
limitations, new methodologies based on the development of biosensors are looking for inexpensive alternatives that not only minimize bioassays time but increase sensitivity and reduce the limits of detection (LOD) in multiple applications [2, 3]. Nanobioconjugates are hybrid nano(bio)materials that result from the integration between nanomaterials and biomolecules [4]. Immobilization of hundreds of active biomolecules and tags on top of n
Data Loading...
