Electrochemical studies of V 2 O 5 /GOx for glucose detection
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Bull Mater Sci (2020)43:283 https://doi.org/10.1007/s12034-020-02268-3
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Electrochemical studies of V2O5/GOx for glucose detection F A DE SOUZA, R C F DA ROCHA, N S VIEIRA, D T CESTAROLLI and E M GUERRA* Departamento de Quı´mica, Biotecnologia e Engenharia de Bioprocessos, Ouro Branco CEP 36420-000, Brazil *Author for correspondence ([email protected]) MS received 23 May 2020; accepted 22 July 2020 Abstract. A component of biosensor was prepared based on vanadium pentoxide xerogel (V2O5) and glucose oxidase (GOx). GOx was immobilized on V2O5 to obtain V2O5/GOx thin film. V2O5/GOx thin film was deposited onto an indiumtin-oxide-coated polyethylene terephthalate film as substrate. The immobilization of the enzyme GOx on V2O5 was crosslinked by covalent bonds. After immobilization of GOx, an increase in total charge was observed. The rugosity factor indicated a 0.125 of electrochemically active surface of V2O5/GOx with low porosity. V2O5/GOx thin film presented a sensibility in different concentrations of glucose as well as a good linear correlation. Keywords.
1.
Glucose; biosensor; V2O5/GOx; electrochemical studies.
Introduction
In recent years, the biotechnology has shown a breakthrough in the processing and development of new materials. In the field of clinical medicine, chemistry and industrial processes, for example, the scientific studies have had a great need to identify and quantify metabolites quickly, inexpensively and reliably and have specificity for the determination of substances in biological fluids. Thus, the development of biosensors became fundamental [1,2]. The necessity for determination of substances in biological fluids, such as blood glucose, cholesterol, lactate, urea, creatine, hemoglobin, has led to the demand for methods that have high selectivity, reliability and inexpensive. Besides, a real-time analyses are explored then the application of biosensors became necessary, especially in clinical analysis [3,4]. Blood glucose monitoring is of utmost importance for diabetes control. According to the International Diabetes Federation (IDF) [5], diabetes is a chronic disease that occurs when the pancreas is no longer able to make insulin, or when the body cannot make good use of the insulin it produces. Besides, insulin is a hormone produced by the pancreas, which acts as a key that allows food glucose to pass from the bloodstream to the body’s cells, producing energy. The non-production of insulin or its effective use leads to elevated blood glucose (known as hyperglycemia), long-term serious damage to the body. Commercially available glucometers require greater sensitivity and lower costs. Currently, there are several materials proposed in the literature that help reduce cost, increase sensitivity and have conductive properties, such as some oxides such as vanadium pentoxide (V2O5) [3], tungsten trioxide (WO3) [4,6], zinc oxide (ZnO) [7], V2O5 nanowires
[8] and titanium dioxide (TiO2) [9]. It is possible to highlight V2O5, whi
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