Electrospun Fibers for Controlled Release of Nanoparticle-Assisted Phage Therapy Treatment of Topical Wounds
- PDF / 1,061,423 Bytes
- 7 Pages / 432 x 648 pts Page_size
- 43 Downloads / 294 Views
MRS Advances © 2018 Materials Research Society DOI: 10.1557/adv.2018.483
Electrospun Fibers for Controlled Release of Nanoparticle-Assisted Phage Therapy Treatment of Topical Wounds Jessica M. Andriolo,1,2 Nathan J. Sutton,1,2 John P. Murphy,2 Lane G. Huston,1,2 Emily A. Kooistra-Manning,2 Robert F. West,2 Marisa L. Pedulla,3 M. Katie Hailer,2,4 and Jack L. Skinner1,2 1
Mechanical Engineering, Montana Tech, 1300 West Park Street, Butte, MT 59701 Montana Tech Nanotechnology Laboratory, Montana Tech, 1300 West Park Street, Butte, MT 59701 3 Biological Sciences, Montana Tech, 1300 West Park Street, Butte, MT 59701 4 Chemistry and Geochemistry, Montana Tech, 1300 West Park Street, Butte, MT 59701
2
ABSTRACT
Bacterial cultures exposed to iron-doped apatite nanoparticles (IDANPs) prior to the introduction of antagonistic viruses experience up to 2.3 times the bacterial destruction observed in control cultures. Maximum antibacterial activity of these bacteria-specific viruses, or phage, occurs after bacterial cultures have been exposed to IDANPs for 1 hr prior to phage introduction, demonstrating that IDANP-assisted phage therapy would not be straight forward, but would instead require controlled time release of IDANPs and phage. These findings motivated the design of an electrospun nanofiber mesh treatment delivery system that allows burst release of IDANPs, followed by slow, consistent release of phage for treatment of topical bacterial infections. IDANPs resemble hydroxyapatite, a biocompatible mineral analogous to the inorganic constituent of mammalian bone, which has been approved by the Food and Drug Administration for many biomedical purposes. The composite nanofiber mesh was designed for IDANP-assisted phage therapy treatment of topical wounds and consists of a superficial, rapid release layer of polyethylene oxide (PEO) fibers doped with IDANPs, followed by inner, coaxial polycaprolactone / polyethylene glycol (PCL/PEG) blended polymer fiber layer for slower phage delivery. Our investigations have established that IDANP-doped PEO fibers are effective vehicles for dissemination of IDANPs for bacterial exposure and resultant increased bacterial death by phage. In this work, slower delivery of the phage behind IDANPs was accomplished using coaxial, electrospun fibers composed of PCL/PEG polymer blend.
Downloaded from https://www.cambridge.org/core. University of Arizona, on 26 Jul 2018 at 08:38:20, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.1557/adv.2018.483
INTRODUCTION It is well known that bacterial strains have become increasingly resistant to traditional antibiotic therapies. The Centers for Disease Control and prevention estimate that at least 2 million people in the United States become infected with antibiotic-resistant bacteria, and at least 23,000 people die each year as a direct result of those infections [1]. As an alternative to traditional antibiotics, bacterial viruses (phages) capable of exponential bacterial destruction h
Data Loading...
