Endometrial regenerative cells: A novel stem cell population
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Endometrial regenerative cells: A novel stem cell population Xiaolong Meng*1, Thomas E Ichim2, Jie Zhong1, Andrea Rogers1, Zhenglian Yin1, James Jackson1, Hao Wang3, Wei Ge3, Vladimir Bogin2, Kyle W Chan2, Bernard Thébaud4 and Neil H Riordan1,2 Address: 1Bio-Communications Research Institute, Wichita, USA, 2Medistem Laboratories Inc, Tempe, USA, 3Department of Surgery, University of Western Ontario, London, Canada and 4Department of Pediatrics, University of Alberta, Edmonton, Canada Email: Xiaolong Meng* - [email protected]; Thomas E Ichim - [email protected]; Jie Zhong - [email protected]; Andrea Rogers - [email protected]; Zhenglian Yin - [email protected]; James Jackson - [email protected]; Hao Wang - [email protected]; Wei Ge - [email protected]; Vladimir Bogin - [email protected]; Kyle W Chan - [email protected]; Bernard Thébaud - [email protected]; Neil H Riordan - [email protected] * Corresponding author
Published: 15 November 2007 Journal of Translational Medicine 2007, 5:57
doi:10.1186/1479-5876-5-57
Received: 26 September 2007 Accepted: 15 November 2007
This article is available from: http://www.translational-medicine.com/content/5/1/57 © 2007 Meng et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Angiogenesis is a critical component of the proliferative endometrial phase of the menstrual cycle. Thus, we hypothesized that a stem cell-like population exist and can be isolated from menstrual blood. Mononuclear cells collected from the menstrual blood contained a subpopulation of adherent cells which could be maintained in tissue culture for >68 doublings and retained expression of the markers CD9, CD29, CD41a, CD44, CD59, CD73, CD90 and CD105, without karyotypic abnormalities. Proliferative rate of the cells was significantly higher than control umbilical cord derived mesenchymal stem cells, with doubling occurring every 19.4 hours. These cells, which we termed "Endometrial Regenerative Cells" (ERC) were capable of differentiating into 9 lineages: cardiomyocytic, respiratory epithelial, neurocytic, myocytic, endothelial, pancreatic, hepatic, adipocytic, and osteogenic. Additionally, ERC produced MMP3, MMP10, GM-CSF, angiopoietin-2 and PDGF-BB at 10–100,000 fold higher levels than two control cord blood derived mesenchymal stem cell lines. Given the ease of extraction and pluripotency of this cell population, we propose ERC as a novel alternative to current stem cells sources.
Introduction Stem cells are undifferentiated cells that can replicate themselves without differentiating, and under specific conditions can differentiate into various specialized cell types. Stem cell therapy holds tremendous promise for repair and/or regeneration of aging and damaged tissue. Broadly speaking, stem cel
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