Enhanced detoxification of exogenous toluene gas in transgenic Ardisia pusilla expressing AtNDPK2 gene
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RESEARCH REPORT
Enhanced detoxification of exogenous toluene gas in transgenic Ardisia pusilla expressing AtNDPK2 gene Chang Ho Ahn1 · Nan‑Sun Kim1 · Ju Young Shin1 · Young Ah Lee1 · Kwang Jin Kim2 · Jeong Ho Kim3 · Pil Man Park1 · Hye Ryun An1 · Yae‑Jin Kim1 · Won Hee Kim1 · Su Young Lee1 Received: 28 April 2020 / Revised: 16 June 2020 / Accepted: 20 July 2020 / Published online: 26 August 2020 © Korean Society for Horticultural Science 2020
Abstract The Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) gene is known to regulate the cellular redox state, and to enhance tolerance to multiple stressors in plants. In this study, we transferred AtNDPK2 under the stress-inducible promoter SWPA2 into Ardisia pusilla to enhance the plants’ ability to detoxify toluene gas. Thirty transgenic A. pusilla lines were confirmed by PCR analysis with AtNDPK2 and NPTII gene-specific primers. In addition, four transgenic A. pusilla lines were further confirmed by Southern blot analysis to verify the gene copy number. Three transgenic lines showed a singlecopy transgene insertion, and one transgenic line had two transgene insertions. To test the gene expression of AtNDPK2 in the transgenic A. pusilla lines exposed to and not exposed to toluene treatment, qRT-PCR analysis was performed. The gene expression of AtNDPK2 in transgenic A. pusilla plants exposed to toluene treatment was significantly higher than that of transgenic plants not exposed to toluene treatment. Finally, we measured toluene removal efficiency of the transgenic and non-transgenic A. pusilla lines exposed to toluene-contaminated air. There was a statistically significant difference between the transgenic and non-transgenic A. pusilla lines at all time points (p
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