Effects of Exogenous Elicitors on Triterpenoids Accumulation and Expression of Farnesyl Diphosphate Synthase Gene in Ino
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pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
Effects of Exogenous Elicitors on Triterpenoids Accumulation and Expression of Farnesyl Diphosphate Synthase Gene in Inonotus obliquus Pei Lin, Zheng-Fei Yan, and Chang-Tian Li
Received: 24 December 2019 / Revised: 12 February 2020 / Accepted: 27 February 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract Exogenous elicitors can influence accumulation of triterpenoids by regulating FPS (farnesyl pyrophosphate synthase) in Inonotus obliquus, such as birch bark (BB), birch rhizosphere soil (BS), Frankia alni (FA), and Rhizobium indigoferae (RI). Among them, the highest yield of biomass (15.6 mg/mL) could be detected by treatment with RI at 2 μg/mL for 10 d, about 2.1-fold of the control. Results showed the significant effect of elicitors on accumulation of triterpenoids by regulating FPS expression level. RI stimulated mycelium to achieve the highest accumulation of triterpenoids about 48.2 mg/g, which was about over 4-fold of the control (9.5 mg/g). Its effect on FPS expression level was greater than that of others, which was about over 10-fold of the control. However, accumulation of triterpenoids and FPS expression level were clearly down-regulated under treatment with BS. In addition, two endogenous factors (H2O2 and NO) in I. obliquus could affect accumulation of triterpenoids by regulating FPS expression level. Effect of RI on H2O2 and NO contents were higher than that of others, about 3.5-fold of the control. The minimum value of H2O2 content was detected Pei Lin School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Ave., Wuxi 21422, China Zheng-Fei Yan* State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave., Wuxi 21422, China Tel/Fax: +86-0510-85326653 E-mail: [email protected] Zheng-Fei Yan, Chang-Tian Li* Engineering Research Center of Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China Tel/Fax: +86-431-84533310 E-mail: [email protected]
by treatment with BS for 10 d, which was about 0.5-fold of the control. RI that assist with CAT (catalase) and SNP (sodium nitroprusside dehydrate) could stimulate triterpenoid contents to achieve highest accumulation, which was about 5.5-fold of the control. Fluctuation of H2O2 and NO content seems to play a pivotal role in accumulation of triterpenoids by regulating FPS expression level. Keywords: exogenous elicitors, Inonotus obliquus, FPS expression, triterpenoids accumulation
1. Introduction Medicinal fungi have an established history of being used in nutritionally functional food as well as traditional oriental therapies [1]. Among them, Inonotus obliquus is a rare medicinal mushroom belonging to the family of Hymenochaetaceae Donk, which grew as parasitism on trunks of living birch in the colder northern climates [2]. I. obliquus can form an irregular shape of sclerotial conk, not fruiting body. Its sclerotia with no side effects, have been confirmed to have vario
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