Heterologous expression of a chimeric gene, OsDST-SRDX , enhanced salt tolerance of transgenic switchgrass ( Panicum vir
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ORIGINAL ARTICLE
Heterologous expression of a chimeric gene, OsDST‑SRDX, enhanced salt tolerance of transgenic switchgrass (Panicum virgatum L.) Huifang Cen1 · Yanrong Liu1 · Dayong Li3 · Kexin Wang1 · Yunwei Zhang1,2 · Wanjun Zhang1,2 Received: 16 January 2020 / Accepted: 20 February 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Overexpression of OsDST-SRDX chimeric gene in switchgrass promotes plant growth and improves the salt tolerance of transgenic switchgrass by improving its antioxidative ability. Abstract Switchgrass (Panicum virgatum L.) is a forage and model feedstock plant. To avoid competing with crops in arable land utilization, improving salt tolerance of switchgrass is required to use marginal saline land for switchgrass production. To improve salt tolerance of switchgrass, a chimeric DROUGHT AND SALT TOLERANCE (DST) gene OsDST-SRDX was constructed using the Chimeric REpressor gene-Silencing Technology (CRES-T), and introduced into switchgrass genome by Agrobacterium-mediated transformation. Compared to wild-type (WT) plants, OsDST-SRDX transgenic (TG) switchgrass plants showed wider leaves and thicker stems. They performed better under salt stress, had higher relative leaf water content, lower electrolyte leakage and lower malondialdehyde (MDA) content, and accumulated less Na+ and more K+ than WT controls. The transgenic plants had also higher activities of antioxidant enzymes associated with suppressed expressing of genes in H2O2 homeostasis, including glutathione S-transferase (GST2, GST6), cytochrome P450, peroxidase 24 precursor, and induced expressing of CATand SOD under salt stress to eliminate excess H 2O2. Our results indicate that overexpression of the chimeric gene OsDST-SRDX improves salt tolerance of switchgrass, a C4 biofuel crop. Keywords Switchgrass · Chimeric REpressor gene-silencing technology (CRES-T) · DST · SRDX domain · Salt tolerance
Introduction
Communicated by Prakash P. Kumar. Huifang Cen and Yanrong Liu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02526-y) contains supplementary material, which is available to authorized users.
Switchgrass (Panicum virgatum L.) is a perennial C 4 warmseason grass and a biomass feedstock plant, which is native to North America (Sage et al. 2015). Because of its high biomass yield and low cash cost in maintenance and production, the U.S. Department of Energy has identified switchgrass as an important biofuel crop (Casler et al. 2007; Sanderson et al. 1996; Vogel et al. 2010). However, to get higher yield in marginal saline land, the salt tolerance of switchgrass
* Wanjun Zhang [email protected]
Yunwei Zhang [email protected]
Huifang Cen [email protected]
1
Yanrong Liu [email protected]
College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
2
Dayong Li [email protected]
National Energy R&D Center for Biomass (NECB),
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