Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probio
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RESEARCH NOTE
Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide‑induced inflammatory bowel disease Ni Nengah Dwi Fatmawati1* , Kazuyoshi Gotoh2, I. Putu Bayu Mayura1,2, Komang Ayu Nocianitri3, Gede Ngurah Rsi Suwardana1, Ni Luh Gede Yoni Komalasari4,5, Yan Ramona6, Masakiyo Sakaguchi4, Osamu Matsushita2 and I. Nengah Sujaya7
Abstract Objective: Weissella confusa F213 (WCF213) and Lactobacillus rhamnosus FBB81 (LrFBB81) are two probiotic candidates isolated from humans in our previous study. Their functional activity on the mucosal barrier has not yet been adequately investigated. Therefore, the objective of this study was to investigate the effect of these strains on maintaining mucosal integrity in vitro. Caco-2 cell monolayers were pretreated with WCF213 and LrFBB81 before being exposed to hydrogen peroxide. The integrity of mucosal cells was evaluated by measuring the transepithelial resistance (TER), flux of FITC-labelled dextran, and ZO-1 protein distribution with the help of an immunofluorescence method. Results: WCF213 was found to significantly maintain the TER better than the control hydrogen peroxide-treated cells (p
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