Lactobacillus plantarum BSGP201683 Improves the Intestinal Barrier of Giant Panda Microbiota-Associated Mouse Infected b
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Lactobacillus plantarum BSGP201683 Improves the Intestinal Barrier of Giant Panda Microbiota-Associated Mouse Infected by Enterotoxigenic Escherichia coli K88 Yi Zhou 1 & Xueqin Ni 1 & Ling Duan 2 & Lili Niu 3 & Qian Liu 1 & Yan Zeng 1 & Qiang Wang 3 & Jie Wang 1 & Abdul Khalique 1 & Kangcheng Pan 1 & Bo Jing 1 & Dong Zeng 1 Accepted: 21 October 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Giant pandas often suffered from gastrointestinal disease, especially the captive sub-adult one. Our study aims to investigate whether L. plantarum G83, a good panda-derived probiotic, can improve the intestinal barrier against the enterotoxigenic Escherichia coli K88 (E. coli K88) infection in giant panda microbiota-associated mice (GPAM). We treated SPF mice with antibiotics cocktail and transplanted the giant panda intestinal microbiota to set up a GPAM. Our results demonstrated that the microbiota of GPAM changed over time and was relatively stable in the short-term experiment (2–4 weeks). Whereafter, the GPAM pretreated with L. plantarum G83 for 15 days and infected with enterotoxigenic E. coli K88. The result indicated that the number of Bifidobacteria spp. increased in GPAM-G and GPAM-GE groups; the Lactobacillus spp. only increased in the GPAM-G group. Although the abundance of Enterobacteriaceae spp. only decreased in the GPAM-G group, the copy number of Escherichia coli in the GPAM-E group was significantly lower than that in the other groups. Meanwhile, the L. plantarum G83-induced alteration of microbiota could increase the mRNA expression of Claudin-1, Zo-1, and Occludin-1 in the GPAM-G group in the ileum; only Occludin-1 was increased in the GPAM-GE group. The sIgA in the ileum showed a positive response, also the result of body weight and histology in both the GPAM-G and GPAM-GE group. These results indicated that the L. plantarum G83 could improve the intestinal barrier to defense the enterotoxigenic E. coli K88 invasion. Keywords Giant panda . Lactobacillus . Fecal microbiota transplantation . Intestinal microflora . Intestinal barrier
Introduction Gastrointestinal disease is one of the leading causes of death in giant panda, especially in sub-adult panda [1, 2]. Based on our review, the Enterobacteriaceae (especially EscherichiaShigella) dominates the intestinal microbiota of the panda ranging from 29.26 to 53.33% [3–5], and more than 50 pathogen strains were isolated from the gastrointestinal tract of
Yi Zhoua, Xueqin Nia, and Ling Duan are Joint first authors * Dong Zeng [email protected] 1
Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2
Qu Country Extension Station for Animal Husbandry Technology, Dazhou 635299, Sichuan, China
3
Chengdu Wildlife Institute, Chengdu Zoo, Chengdu 610081, Sichuan, China
panda and more than 70% of strains belonging to Enterobacteriaceae [6]. Additionally, the sub-adult is prone to intestinal microbiota disorder and disease infection during the dietary hab
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