Enhancing electrochemical sensing for catechol by biomimetic oxidase covalently functionalized graphene oxide
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RESEARCH PAPER
Enhancing electrochemical sensing for catechol by biomimetic oxidase covalently functionalized graphene oxide Xia Jiaojiao1 · Wang Pengyun1 · Zou Bin1 · Adesanya Idowu Onyinye1 Received: 18 May 2020 / Accepted: 9 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Catechol level is an important indicator for evaluating the quality of tea. Therefore, the exploration of a simple and efficient quantitative detection method for catechol has an important significance. In this study, functionalized graphene oxide was synthesized by chemically modifying the surface of graphene oxide. The prepared carrier was covalently combined with biomimetic oxidase iron porphyrin (FePP, the active center of horseradish peroxidase). Ionic liquid as covalent coupling agents was designed as electronic bridge between biomimetic oxidase and graphene oxide. The novel biomimetic biosensor provided a detection range of 50.0–1600.0 μmol/L by modulating under the optimal conditions of the reaction system (FePP concentration is 1.5 × 10–3 mol/L, pH 3.0, Nafion solution dosage 1% and temperature 25 °C), the detection limit is 0.09 μmol/L. The biosensor has excellent stability, repeatability and reproducibility, and is expected to be applied to the rapid detection of catechol in actual tea sample.. Graphic abstract
Keywords Biomimetic oxidase · Iron porphyrin · Graphene oxide · Functionalized surface · Catechol
Introduction * Zou Bin [email protected] 1
School of Food and Biological Engineering, Jiangsu University, No.301 Xuefu Road, Zhenjiang 212013, China
In light of anti-oxidation, anti-virus, prevention of the accumulation of harmful proteins and protection of brain cells, catechol, a natural component of tea has attracted much
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attention recently. It has become one of the important indicators for judging tea quality [1]. Developing a simple and efficient quantitative detection method for catechol is thus of important significance for the evaluation of comprehensive quality of tea and human health. Extensive methods have been used in recent years to detect catechol, including spectrophotometry, flow injection and high-performance liquid chromatography [2]. These different techniques are reliable, but their cumbersome and costly operation, time-detection is a major technical problem hindering their large-scale application. Therefore, a fast and convenient method is desirable for practical application. Nowadays, efficient electrochemical biosensor technology is a potential alternative to the detection of catechol [3–5]. Albayati et al. [6] introduced a novel electrode for catechol detection by covalent immobilization of laccase (Lac) on a glassy carbon electrode modified by gold nanoparticles (AuNPs) and carboxylated multiwalled carbon nanotubes(cMWCNTs). The catechol detection limit was 12.26 μM. Similar low detection limit of 3.75 μM was also reported using active center iron porphyrin (FePP) of the horseradish peroxidase (HRP) instead of above laccase in o
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