Epitope Mapping Protocols

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Par

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Johan Rockberg Johan Nilvebrant Editors

Epitope Mapping Protocols Third Edition

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Epitope Mapping Protocols Third Edition

Edited by

Johan Rockberg KTH School of Engineering Sciences in Chemistry, Biotechnology and Health, Protein Technology, Stockholm, Sweden

Johan Nilvebrant KTH School of Engineering Sciences in Chemistry, Biotechnology and Health, Protein Engineering, Stockholm, Sweden; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada

Editors Johan Rockberg KTH School of Engineering Sciences in Chemistry Biotechnology and Health Protein Technology Stockholm, Sweden

Johan Nilvebrant KTH School of Engineering Sciences in Chemistry Biotechnology and Health Protein Engineering Stockholm, Sweden Donnelly Centre for Cellular and Biomolecular Research University of Toronto Toronto, ON, Canada

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7839-7 ISBN 978-1-4939-7841-0 (eBook) https://doi.org/10.1007/978-1-4939-7841-0 Library of Congress Control Number: 2018940275 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Antibodies are among the most valued molecules within life science today and are routinely used for making decisions at universities, industries, and hospitals affecting directions of research, investments, and quality of life. A lot o