Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1
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ORIGINAL PAPER
Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1 Mehdi Moghanibashi & Ferdous Rastgar Jazii & Zahra-Soheila Soheili & Maryam Zare & Aliasghar Karkhane & Kazem Parivar & Parisa Mohamadynejad Received: 9 August 2012 / Revised: 26 February 2013 / Accepted: 14 March 2013 / Published online: 29 March 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Nuclear pore complex (NPC) is the only corridor for macromolecules exchange between nucleus and cytoplasm. NPC and its components, nucleoporins, play important role in the diverse physiological processes including macromolecule exchange, chromosome segregation, apoptosis and gene expression. Recent reports also suggest involvement of nucleoporins in carcinogenesis. Applying proteomics, we analyzed expression pattern of the NPC components in a newly established esophageal cancer cell line from Persia (Iran), the high-risk region for esophageal cancer. Our results indicate overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1. Among these proteins, Hsc70 and eIF5A-1 are in direct interaction with NPC and involved in the nucleocytoplasmic exchange. Hsc70 plays a critical role as a chaperone in the formation of a cargo–receptor complex in nucleocytoplasmic transport. On the other hand, it is an NPC-associated protein that binds to nucleoporins and contributes in recycling of the nucleocytoplasmic transport receptors in mammals and affects transport of proteins between nucleus and cytoplasm. The M. Moghanibashi (*) : K. Parivar Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran e-mail: [email protected] F. Rastgar Jazii : Z.-S. Soheili : A. Karkhane Department of Biochemistry, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran F. Rastgar Jazii Department of Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada M. Zare Department of Biology, Payam-Noor University, Tehran, Iran P. Mohamadynejad Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
other nuclear pore interacting protein: eIF5A-1 binds to the several nucleoporins and participates in nucleocytoplasmic transport. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis. Keywords Nuclear pore complex (NPC) . Proteomics . Esophageal squamous cell carcinoma (ESCC) . Esophageal cancer . Hsc70 . eIF5A-1
Introduction Eukaryotic cells have compartmentalized their genetic material inside the nucleus surrounded by a nuclear envelope. The only corridors for nucleocytoplasmic exchange of macromolecules, ribosomal subunits, and flow of information such as mRNA are nuclear pore complexes (NPCs) (Cole and Scarcelli 2006; Terry et al. 2007). Localized in the nuclear envelope NPCs are formed by multiple copies of approximately 30 different proteins known as nucleoporins (Nup
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