HtrA1 expression associated with the occurrence and development of esophageal cancer
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WORLD JOURNAL OF SURGICAL ONCOLOGY
RESEARCH
Open Access
HtrA1 expression associated with the occurrence and development of esophageal cancer Youtao Yu1*, Wenlong Shao2,3, Yi Hu4,5, Jingyan Zhang1, Hao Song1 and Zhi-hua Zhu4,5*
Abstract Background: The purposes of this study were to measure both the mRNA and protein expression levels of high-temperature requirement serine peptidase 1 (HtrA1) in human esophageal cancer tissues and their adjacent, comparatively normal esophageal tissues. Methods: The expression levels of HtrA1 mRNA and protein in both tissue types were measured by semi-quantitative RT-PCR (reverse transcription-polymerase chain reaction) and Western blotting. The clinical and pathological correlation between HtrA1 expression levels and the occurrence and development of esophageal cancer was analyzed. Results: The expression levels of HtrA1 mRNA and protein in esophageal carcinoma were significantly lower than the levels expressed in their adjacent normal esophageal tissue (p < 0.05). The more highly undifferentiated esophageal tumor cells expressed lower HtrA1 mRNA and protein expression levels (p < 0.05). Patients with tumors in early pathological stages (I-II) had significantly higher HtrA1 mRNA and protein expression levels than did patients with tumors in mid-to-late pathological stages (III-IV) (p < 0.05). Patients with positive lymph node metastasis had significantly lower HtrA1 mRNA and protein expression levels than did patients with lymph node-negative disease (p < 0.05). Conclusions: HtrA1 expression is associated with the occurrence and development of esophageal cancer. Keywords: Esophageal cancer, HtrA1 expression, Semi-quantitative RT-PCR, SiRNA
Background Multimodality therapy for esophageal cancer has improved patient outcomes and is favored by the current standard of practice for surgical patients [1-3]. Neoadjuvant chemoradiotherapy followed by surgery appears to increase resectability, produce tumor downstaging and improve local control, disease-free survival and overall survival compared to surgery alone [4-7]. However, esophageal cancers have a poor prognosis and a 5-year survival rate of less than 10% [8,9]. This poor outcome is mainly due to frequent and extensive invasion outside of the esophagus or regional lymph node metastasis, which can occur during the early stages of esophageal cancer [10,11]. The occurrence and development of esophageal cancers is the result of the cooperative action of multiple genes. Therefore, searching for * Correspondence: [email protected]; [email protected] 1 Department of Interventional Radiology, The Third Affiliated Hospital of Harbin Medical University, No. 150, Haping Rd, Harbin 150040, China 4 State Key Laboratory of Oncology in South China, Guangzhou, China Full list of author information is available at the end of the article
genes associated with the occurrence and development of esophageal cancer and its metastasis has become a heavily investigated topic in current studies. Examining the genes associated with the occurrence, developme
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