Establishment and biological characteristics of fibroblast cell lines obtained from wild corsac fox
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Establishment and biological characteristics of fibroblast cell lines obtained from wild corsac fox Xihe Li 1,2 & Yunxia Li 1,2 & Xiaojie Yan 1 & Xiaonan Guo 1 & Yongli Song 1 & Baojiang Wu 1 & Siqin Bao 1 & Guifang Cao 2,3 & Jitong Guo 1 & Qingyuan Sun 4 Received: 24 September 2020 / Accepted: 25 October 2020 / Editor: Tetsuji Okamoto # The Author(s) 2020
Introduction Animal genetic resources are basic materials for life science studies and are important resources for the survival and economic development of humanity (Min 2010; Liu 2011; Cheng et al. 2018). Wild animal resources are important components of China’s natural resources (Qu 2018). The low-temperature preservation of animal cells is an effective method for the protection of animal genetic resources and is particularly important in the conservation of endangered animal species (Shang 2018). Isolation and cultivation of fibroblasts from different animal tissues for the establishment of fibroblast cell lines are commonly used methods for the preservation of live tissue genetic materials. These cell materials can be stored for “half-permanent” in a – 196°C liquid nitrogen environment (Daorna et al. 2013). Preserved animal genetic resources can be used for animal cloning to revive corresponding species and provide materials for experiments in the fields of stem cells, genetic engineering, cell engineering, and molecular biology (Min 2010). Corsac fox (Vulpes corsac) is mainly lhabitated in Central Asia and is the smallest species among foxes Yunxia Li and Xiaojie Yan contributed equally to this work. * Xihe Li [email protected] 1
Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot 010070, China
2
Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot 011517, People’s Republic of China
3
College of Veterinary Science, Inner Mongolia Agricultural University, Hohhot 010018, People’s Republic of China
4
Institute of Zoology, Chinese Academy of Science, Beijing 100101, People’s Republic of China
(Zhao et al. 2016b) (Zhao et al. 2016a, b). Previous studies on corsac were mainly focused on their genetics and systematic taxonomy (Graphodatsky et al. 2008; Zhao et al. 2016a; Shang et al. 2017), biochemistry, and physiology (I V et al. 1900; Pozio et al. 1992; Tang et al. 2001; Kuzmin et al. 2004; Botvinkin et al. 2008; Odontsetseg et al. 2009; Ito et al. 2013), as well as ecological distribution (Mal'kova 2000; Tang et al. 2004). Presently, there is no report available on the establishment of fibroblast cell lines in corsac fox and its biological characteristics.
Results and discussion One female corsac fox from Horinger County of Inner Mongolia was used to obtain the required tissue samples. The prepared tissue samples were cut into 0.1–0.5 mm3 tissue blocks and were placed at the bottom of T25 culture flasks (Corning, Shanghai, China). Six- to 8-mL culture medium (MEM-Alpha containing 10% FBS and 1% P/S) (Gibco, Shanghai, China
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