Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle ( Eretmochelys imbricata )
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ficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata) Tomokazu Fukuda & Jun Kurita & Tomomi Saito & Kei Yuasa & Masanobu Kurita & Kenichiro Donai & Hiroshi Nitto & Makoto Soichi & Katsuhiko Nishimori & Takafumi Uchida & Emiko Isogai & Manabu Onuma & Hideko Sone & Norihisa Oseko & Miho Inoue-Murayama
Received: 30 July 2012 / Accepted: 15 October 2012 / Published online: 13 November 2012 # The Society for In Vitro Biology 2012
Abstract The hawksbill sea turtle (Eretmochelys imbricata) is a critically endangered species at a risk of extinction. Preservation of the genomic and cellular information of endangered animals is important for future genetic and biological studies. Here, we report the efficient T. Fukuda (*) : K. Donai : K. Nishimori : T. Uchida : E. Isogai Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi Aobaku, Sendai 981-8555, Japan e-mail: [email protected] J. Kurita : K. Yuasa : N. Oseko National Research Institute of Aquaculture, Fisheries Research Agency of Japan, Watarainakatuhamaura, Mie, Japan T. Saito Usa Marine Biological Institute, Kochi University, Kami, Kochi, Japan M. Kurita : H. Nitto : M. Soichi Port of Nagoya Public Aquarium, Nagoya, Aichi, Japan
establishment of primary fibroblast cultures from skin tissue of the hawksbill sea turtle. We succeeded in establishing 19 primary cultures from 20 hawksbill sea turtle individuals (a success rate of 95%). These cells exhibited a fibroblast-like morphology and grew optimally at a temperature of 26°C, but experienced a loss of viability when cultured at 37°C. Chromosomal analysis using the primary cells derived here revealed that hawksbill sea turtles have a 2n056 karyotype. Furthermore, we showed that our primary cell cultures are free of several fish-related viruses, and this finding is important for preservation purposes. To our knowledge, this report is the first to describe primary cell cultures established from normal tissues of the hawksbill sea turtle. The results will contribute to the preservation of biodiversity, especially for the sea turtles that are critically endangered owing to human activities. Keywords Hawksbill sea turtle . Cultured cells . Critically endangered animals . Chromosome analysis
Introduction M. Onuma Ecological Genetics Analysis Section, Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies of Japan, Tsukuba, Japan H. Sone Health Risk Research Section, Center for Environmental Risk Research, National Institute for Environmental Studies, Tsukuba, Japan M. Inoue-Murayama Wildlife Research Center of Kyoto University, Kyoto, Japan
The hawksbill sea turtle (Eretmochelys imbricata) is classified by the International Union for the Conservation of Nature as critically endangered and at a risk of extinction (Groombridge 1982). Populations of hawksbill sea turtle have declined dramatically in recent years because of hunting and the loss of egg-laying sites (Bass et al. 1996). Hunting
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